Genetic characterization

New polymorphic microsatellite markers for the Korean manila clam (Ruditapes philippinarum) and their application to wild populations

E. M. Kim, An, H. S., Kang, J. H., An, C. M., Dong, C. M., Hong, Y. K., and Park, J. Y., New polymorphic microsatellite markers for the Korean manila clam (Ruditapes philippinarum) and their application to wild populations, vol. 13, pp. 8163-8173, 2014.

Manila clam (Ruditapes philippinarum) is a valuable and intensively exploited shellfish species in Korea. Despite its importance, information on its genetic background is scarce. For the genetic characterization of R. philippinarum, expressed sequence tag-derived microsatellite markers were developed using next-generation sequencing. A total of 5879 tandem repeats containing di- to hexanucleotide repeat motifs were obtained from 236,746 reads (mean = 413 bp).

Genetic characterization of Red Junglefowl (Gallus gallus), Thai indigenous chicken (Gallus domesticus), and two commercial lines using selective functional genes compared to microsatellite markers

P. Akaboot, Duangjinda, M., Phasuk, Y., Kaenchan, C., and Chinchiyanond, W., Genetic characterization of Red Junglefowl (Gallus gallus), Thai indigenous chicken (Gallus domesticus), and two commercial lines using selective functional genes compared to microsatellite markers, vol. 11, pp. 1881-1890, 2012.

Genetic characterization among Red Junglefowl (GS, Gallus gallus spadiceus), Thai indigenous chicken (TIC, Gallus domesticus) and commercial lines has been widely used for studies of genealogical origin, genetic diversity, and effects of selection. We compared the efficiency of genetic characterization of chicken populations that had been under different intensities of selection using selective functional gene versus microsatellite marker analyses.

Genetic characterization of pea (Pisum sativum) germplasm from Turkey using morphological and SSR markers

G. Sarıkamış, Yanmaz, R., Ermiş, S., Bakır, M., Bakır, M., and Yüksel, C., Genetic characterization of pea (Pisum sativum) germplasm from Turkey using morphological and SSR markers, vol. 9, pp. 591-600, 2010.

The need for the conservation of plant genetic resources has been widely accepted. Germplasm characterization and evaluation yield information for more efficient utilization of these valuable resources. The aim of the present study was to characterize the pea germplasm conserved at the Aegean Agricultural Research Institute of Turkey using morphological and simple sequence repeat (SSR)-based molecular approaches.

Genetic characterization of heat tolerant tomato (Solanum lycopersicon) genotypes by SRAP and RAPD markers

N. Comlekcioglu, Simsek, O., Boncuk, M., and Aka-Kacar, Y., Genetic characterization of heat tolerant tomato (Solanum lycopersicon) genotypes by SRAP and RAPD markers, vol. 9, pp. 2263-2274, 2010.

We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable Research and Development Center, and a sample of S. pimpinellifolium were genotyped with RAPD and SRAP markers. Eleven SRAP primer combinations were used and 66 bands were scored.

Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey

G. Sarıkamış, Yaşar, F., Bakır, M., Bakır, M., Kazan, K., and Ergül, A., Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey, vol. 8, pp. 880-887, 2009.

Green bean genotypes collected from eastern Turkey were characterized using simple sequence repeat (SSR) markers and morphological traits. Among 12 SSR markers, 10 produced successful amplifications and revealed DNA polymorphisms that were subsequently used to assess genetic relatedness of the genotypes. Based on the number of alleles generated and the probability of identity values, the most informative SSR loci were PVGLND5, PVMEIG, PV-ag001, and PV-ag004.

Genetic characterization of European-Zebu composite bovine using RFLP markers

E. Perez Marson, Ferraz, J. Bento Ster, Meirelles, F. Vieira, Balieiro, J. César de, Eler, J. Pereira, Figueiredo, L. Gustavo Gi, and Mourão, G. Barreto, Genetic characterization of European-Zebu composite bovine using RFLP markers, vol. 4, pp. 496-505, 2005.

A population of 370 European-Zebu composite beef heifers, consisting of six different breed compositions (A-F), were characterized genetically, using RFLP markers of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes. Our objectives were to genetically characterize this population and to determine the structure and the genetic variability of this hybrid herd. The genotypes were determined through PCR, followed by digestion with restriction endonucleases.

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