Genetic diversity

Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq

X. F. Zheng, You, Y. N., Diao, Y., Zheng, X. W., Xie, K. Q., Zhou, M. Q., Hu, Z. L., and Wang, Y. W., Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq, vol. 14, pp. 11171-11184, 2015.

Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess diversity across cultivated and wild lotus. A total of 105,834 uni-contigs were produced with an average read length of 722 bp. Exactly 11,178 genic-SSR loci were identified in 9523 uni-contigs.

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