Genetic diversity

Analysis of the genetic diversity of super sweet corn inbred lines using SSR and SSAP markers

W. R. Ko, Sa, K. J., Roy, N. S., Choi, H. - J., Lee, J. K., Ko, W. R., Sa, K. J., Roy, N. S., Choi, H. - J., Lee, J. K., Ko, W. R., Sa, K. J., Roy, N. S., Choi, H. - J., and Lee, J. K., Analysis of the genetic diversity of super sweet corn inbred lines using SSR and SSAP markers, vol. 15, p. -, 2016.

In this study, we compared the efficiency of simple sequence repeat (SSR) and sequence specific amplified polymorphism (SSAP) markers for analyzing genetic diversity, genetic relationships, and population structure of 87 super sweet corn inbred lines from different origins. SSR markers showed higher average gene diversity and Shannon’s information index than SSAP markers. To assess genetic relationships and characterize inbred lines using SSR and SSAP markers, genetic similarity (GS) matrices were constructed.

Haplo-block structure of Southern African village chicken populations inferred using genome-wide SNP data

K. S. Khanyile, Dzomba, E. F., and Muchadeyi, F. C., Haplo-block structure of Southern African village chicken populations inferred using genome-wide SNP data, vol. 14, pp. 12276-12287, 2015.

This study investigated the haplo-block structure, haplotype sharing, and diversity in extensively raised chicken populations of Southern Africa. Two hundred ninety village chickens from Malawi (N = 30), South Africa (N = 132), and Zimbabwe (N = 128) were included in the study, from which 649, 2104, and 2442 haplo-blocks were observed, respectively. The majority of haplo-blocks were smaller than 25 kb in size and only five blocks were more than 2000 kb in size.

Development of SNP markers and their application for genetic diversity analysis in the oil palm (Elaeis guineensis)

P. W. Ong, Maizura, I., Abdullah, N. A. P., Rafii, M. Y., Ooi, L. C. L., Low, E. T. L., and Singh, R., Development of SNP markers and their application for genetic diversity analysis in the oil palm (Elaeis guineensis), vol. 14, pp. 12205-12216, 2015.

The genetic evaluation of oil palm germplasm collections is required for insight into the variability among populations. The information obtained is also useful for incorporating new genetic materials into current breeding programs. Single nucleotide polymorphisms (SNPs) have been widely used in many plant genetic studies due to the availability of large numbers of genomic sequences and expressed sequence tags. The present study examined 219 oil palms collected from two natural Angolan populations, a few hundred kilometers apart.

Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae)

J. M. Lai, Tsai, C. C., Yen, C. R., Ko, Y. Z., Chen, S. R., Weng, I. S., Lin, Y. S., and Chiang, Y. C., Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae), vol. 14, pp. 13013-13021, 2015.

Syzygium samarangense (Blume) Merr. & Perry (wax apple) is an important commercial fruit tree in Southeast Asia. Here, microsatellite markers were developed to evaluate genetic diversity and distinguish cultivars in this species. In total, 161 microsatellite loci with sufficient flanking sequences to design primer sets were isolated from wax apple using a magnetic bead-enrichment method. Fifty-eight primer sets were designed based on the flanking sequences of each single sequence repeat (SSR) locus and were tested using 14 wax apple cultivars/lines.

Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers

L. Wei, Shao, W. W., Zhou, H. B., Ping, J., Li, L. M., and Zhang, Y. P., Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers, vol. 14, pp. 14119-14122, 2015.

Twelve polymorphic microsatellite loci were isolated in the Japanese gecko, Gekko japonicus. We genotyped one population from Wenzhou, Zhejiang Province, China (N = 36). The mean number of observed alleles per locus was 7.3 (range 4 to 13). Observed and expected heterozygosity values ranged from 0.200 to 0.944 and from 0.395 to 0.797, respectively. One locus (GJ20) showed significant departure from Hardy-Weinberg equilibrium; no linkage disequilibrium was found between any two loci.

Genetic diversity and relationship analysis of Gossypium arboreum accessions

F. Liu, Zhou, Z. L., Wang, C. Y., Wang, Y. H., Cai, X. Y., Wang, X. X., Zhang, Z. S., and Wang, K. B., Genetic diversity and relationship analysis of Gossypium arboreum accessions, vol. 14, pp. 14522-14529, 2015.

Simple sequence repeat techniques were used to identify the genetic diversity of 101 Gossypium arboreum accessions collected from India, Vietnam, and the southwest of China (Guizhou, Guangxi, and Yunnan provinces). Twenty-six pairs of SSR primers produced a total of 103 polymorphic loci with an average of 3.96 polymorphic loci per primer. The average of the effective number of alleles, Nei’s gene diversity, and Shannon’s information index were 0.59, 0.2835, and 0.4361, respectively. The diversity varied among different geographic regions.

Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species

Q. H. Gu, Cheng, Q. Q., Li, X. J., and Zhou, C. J., Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species, vol. 14, pp. 15201-15212, 2015.

Bellamya is a widely distributed freshwater snail genus in China; however, its genetic diversity is completely unknown. Sixty-five novel microsatellite loci were isolated and characterized from a microsatellite-enriched library of Bellamya aeruginosa genomic DNA. Most of the 65 loci were successfully amplified. We found high polymorphic information content values for these loci, ranging from 0.235 to 0.892. There were 3 to 12 alleles per locus, and the HE and HO varied from 0.425 to 0.953 and 0.026 to 1.000, respectively.

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

G. A. Luz, Gomes, S. O., Neto, R. B. Araújo, Nascimento, M. S. C. B., and Lima, P. S. C., Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers, vol. 14, pp. 15242-15248, 2015.

Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence.

Genetic diversity in three natural populations of Pitcairnia flammea (l.) John (Bromeliaceae) estimated by ISSR markers

F. B. Souza-Sobreira, Souza, G. B., Rosado, C. C. G., Miranda, F. D., Soares, T. C. B., and Gontijo, A. B. P. L., Genetic diversity in three natural populations of Pitcairnia flammea (l.) John (Bromeliaceae) estimated by ISSR markers, vol. 14, pp. 15892-15901, 2015.

Bromeliads are greatly represented in the Atlantic Forest, although many species are threatened with extinction owing to habitat fragmentation and intense extraction for ornamental purposes. Therefore, it is necessary to conduct studies generating knowledge about genetic diversity and the distribution of this diversity among and within natural populations to establish conservation strategies. These studies can be performed with the use of molecular markers.

Genetic diversity analysis of fruit characteristics of hawthorn germplasm

K. Su, Guo, Y. S., Wang, G., Zhao, Y. H., and Dong, W. X., Genetic diversity analysis of fruit characteristics of hawthorn germplasm, vol. 14, pp. 16012-16017, 2015.

One hundred and six accessions of hawthorn intraspecific resources, from the National Germplasm Repository at Shenyang, were subjected to genetic diversity and principal component analysis based on evaluation data of 15 fruit traits. Results showed that the genetic diversity of hawthorn fruit traits varied. Among the 15 traits, the fruit shape variable coefficient had the most obvious evaluation, followed by fruit surface state, dot color, taste, weight of single fruit, sepal posture, peduncle form, and metula traits.

Pages

Subscribe to Genetic diversity