Genetic diversity

Genetic diversity and taxonomic status of Gymnocypris chilianensis based on the mitochondrial DNA cytochrome b gene

J. P. Zhang, Liu, Z., Zhang, B., Yin, X. Y., Wang, L., Shi, H. N., and Kang, Y. J., Genetic diversity and taxonomic status of Gymnocypris chilianensis based on the mitochondrial DNA cytochrome b gene, vol. 14, pp. 9253-9260, 2015.

In order to study the genetic diversity and taxonomic status of Gymnocypris chilianensis on a molecular level, the mitochondrial DNA cytochrome b gene was sequenced for 74 individuals of G. chilianensis from two locations (Heihe River and Shule River) and 42 individuals of its affinis species Gymnocypris przewalskii.

Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat

M. Ejaz, Qidi, Z., Gaisheng, Z., Na, N., Huiyan, Z., and Qunzhu, W., Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat, vol. 14, pp. 8935-8946, 2015.

Amplified fragment length polymorphism markers were used to assess genetic diversity in 10 male sterile wheat crop lines (hetero-cytoplasm with the same nucleus) in relation to a restorer wheat line. These male sterile lines were evaluated using 64 amplified fragment length polymorphism primer combinations, and 13 primers produced polymorphic bands, generating a total 682 fragments. Of the 682 fragments, 113 were polymorphic. The polymorphic information content and marker index values demonstrated the utility of the primer combinations used in the present study.

Characterization of microsatellite markers and their correlations with growth traits in Mandarin fish (Siniperca chuatsi)

T. L. Yi, Fang, L., Liang, X. F., Sun, L. F., Li, J., Luo, X. N., Guo, W. J., Dou, Y. Q., and Sun, J., Characterization of microsatellite markers and their correlations with growth traits in Mandarin fish (Siniperca chuatsi), vol. 14, pp. 8926-8934, 2015.

Mandarin fish (Siniperca chuatsi) is a traditionally cultured freshwater fish with high commercial value in China. To facilitate marker-assisted selection for genetic improvement of this species, 100 microsatellite markers identified in previous studies were characterized in the 25 largest and 25 smallest individuals. Twenty polymorphic loci were used to genotype 200 individuals, and the associations between their genotypes and growth traits were examined.

Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi

C. F. Sun, Ye, X., Tian, Y. Y., and Dong, J. J., Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi, vol. 14, pp. 9343-9352, 2015.

In order to provide genetic information for the selective breeding of Siniperca chuatsi, 14 microsatellite DNA loci were used to evaluate the genetic diversity and structure of four farmed populations and one wild population in China. The four cultivated populations were Foshan (FS), Jiangmen (JM), Nanjing (NJ), and Hongze Lake (HZL), and the wild population was collected from the Hubei HuangGang section of the Yangtze River (HG).

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers

M. P. Liu, Du, H. Y., Zhu, G. P., Fu, D. L., and Tana, W. Y., Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers, vol. 14, pp. 9722-9729, 2015.

Simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) markers were used to evaluate genetic diversity among 22 sweet kernel apricot accessions and 12 cultivars in China to provide information on how to improve the utilization of kernel apricot germplasms. The results showed that 10 pairs of SSR primers screened from 40 primer pairs amplified 43 allelic variants, all of which were polymorphic (100%), and 9 ISSR primers selected from 100 primers amplified 67 allelic variants with 50 polymorphic bands (74.63%).

Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm

R. S. Celestino, Zucchi, M. I., Pinheiro, J. B., Campos, J. B., Pereira, A. A., Bianchini, F. G., Lima, R. N., Arrigoni-Blank, M. F., Alves, P. B., and Blank, A. F., Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm, vol. 14, pp. 9452-9468, 2015.

Due to the economic interests in vetiver, Chrysopogon zizanioides (L.) Roberty, molecular and chemical studies are essential to generate information for its sustainable exploitation. The aim of this study was to undertake a molecular and chemical characterization of vetiver accessions of the active germplasm bank of the Universidade Federal de Sergipe.

Analysis of the genetic diversity of beach plums by simple sequence repeat markers

X. M. Wang, Wu, W. L., Zhang, C. H., Zhang, Y. P., Li, W. L., and Huang, T., Analysis of the genetic diversity of beach plums by simple sequence repeat markers, vol. 14, pp. 9693-9702, 2015.

The purpose of this study was to measure the genetic diversity of wild beach plum and cultivated species, and to determine the species relationships using SSRs markers. An analysis of genetic diversity from ten beach plum germplasms was carried out using 11 simple sequence repeat (SSR) primers selected from 35 primers to generate distinct PCR products. From this plant material, 44 allele variations were detected, with 3-5 alleles identified from each primer.

Genetic diversity of popcorn genotypes using molecular analysis

F. S. Resh, Scapim, C. A., Mangolin, C. A., Machado, M. F. P. S., Amaral, Jr., A. Tdo, Ramos, H. C. C., and Vivas, M., Genetic diversity of popcorn genotypes using molecular analysis, vol. 14, pp. 9829-9840, 2015.

In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were monomorphic and 92 were polymorphic. To calculate the genetic distances among the 26 genotypes, the complements of the Jaccard, Dice, and Rogers and Tanimoto similarity coefficients were used.

Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin

S. O. Bafeel, Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin, vol. 14, pp. 9974-9984, 2015.

The genetic diversity of Saudi locally growing sorghum (Sorghum bicolor) cultivars has not been thoroughly characterized. To understand the genomic patterns of diversification in Saudi sorghum cultivars (N = 7), random amplified polymorphic DNA (RAPD) was used as a rapid, inexpensive method for providing information regarding genomic variability below the species level. Six commercially available primers were initially used to select a single primer based on availability, universality, and its use with standard polymerase chain reaction (PCR) conditions.

Polymorphic microsatellite markers in the traditional Chinese medicinal plant Paris polyphylla var. yunnanensis

Y. Song, Li, M. F., Xu, J., Zhao, Z., and Chen, N. Z., Polymorphic microsatellite markers in the traditional Chinese medicinal plant Paris polyphylla var. yunnanensis, vol. 14, pp. 9939-9942, 2015.

Paris polyphylla var. yunnanensis is a traditional Chinese medicinal plant and is listed as vulnerable by the IUCN. This medicinal herb is well known for its analgesic and anti-inflammatory properties, most notably as an ingredient of the hemostatic compound “Yunnan Baiyao”. However, over-exploitation of the plant for economic purposes is pushing the species to the brink of extinction. Therefore, there is an urgent need to develop conservation strategies for this endangered species, for example, through assessment of its genetic structure and diversity.

Pages

Subscribe to Genetic diversity