Genetic diversity

Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium

B. H. Wang, Rong, P., Cai, X. X., Wang, W., Zhu, X. Y., Chen, C. J., Xu, Y. Y., Huang, X. J., Zhuang, Z. M., and Wang, C. B., Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium, vol. 14, pp. 10630-10644, 2015.

Cotton (Gossypium spp) is one of the most economically important crops that provide the world’s most widely used natural fiber. Diseases such as Fusarium wilt and particularly Verticillium wilt seriously affect cotton production, and thus breeding for disease resistance is one of the most important goals of cotton breeding programs. Currently, potential exists to improve disease resistance in cultivated cotton.

Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb

W. H. Zheng, Zhuo, Y., Liang, L., Ding, W. Y., Liang, L. Y., and Wang, X. F., Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb, vol. 14, pp. 10422-10432, 2015.

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of C. wenyujin, using ran­dom amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers.

Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment

M. Yildiz, Koçak, M., and Baloch, F. S., Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment, vol. 14, pp. 10588-10602, 2015.

Lack of requisite genetic variation in Turkish okra has necessitated the use of different types of markers for estimating the genetic diversity and identifying the source of variation. Transposable elements, present abundantly in plant genomes, generate genomic diversity through their replication and are thus an excellent source of molecular markers. We hypothesized that inter-primer binding site (iPBS)-retrotransposons could be the source of variation because of their genome plasticity nature.

Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq

X. F. Zheng, You, Y. N., Diao, Y., Zheng, X. W., Xie, K. Q., Zhou, M. Q., Hu, Z. L., and Wang, Y. W., Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq, vol. 14, pp. 11171-11184, 2015.

Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess diversity across cultivated and wild lotus. A total of 105,834 uni-contigs were produced with an average read length of 722 bp. Exactly 11,178 genic-SSR loci were identified in 9523 uni-contigs.

Genetic diversity of wild soybean populations in Dongying, China, by simple sequence repeat analysis

Y. H. Wang, Zhang, X. J., and Fan, S. J., Genetic diversity of wild soybean populations in Dongying, China, by simple sequence repeat analysis, vol. 14, pp. 11613-11623, 2015.

Annual wild soybean (Glycine soja Sieb. et Zucc.), the ancestor of cultivated soybean (G. max), is believed to be a potential gene source for further improvement of soybean to cope with environmental stress. In this study, 10 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity and population genetic structure in five wild soybean populations using 195 accessions collected from Dongying, China. Ten SSR markers yielded 90 bands, with an average of nine bands per marker.

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