Genetic diversity

Development of novel chloroplast microsatellite markers for Ginkgo biloba

M. Xu, Xu, L. A., Cao, F. L., Zhang, H. J., and Yu, F. X., Development of novel chloroplast microsatellite markers for Ginkgo biloba, vol. 14, pp. 7715-7720, 2015.

Ginkgo biloba is considered to be a living fossil that can be used to understand the ancient evolutionary history of gymnosperms, but little attention has been given to the study of its population genetics, molecular phylogeography, and genetic resources assessment. Chloro­plast simple sequence repeat (cpSSR) markers are powerful tools for genetic studies of plants. In this study, a total of 30 perfect cpSSRs of Ginkgo were identified and characterized, including di-, tri, tetra-, penta-, and hexanucleotide repeats.

Genetic diversity in Tunisian populations of faba bean (Vicia faba L.) based on morphological traits and molecular markers

I. Z. Backouchi, Aouida, M., Khemiri, N., and Jebara, M., Genetic diversity in Tunisian populations of faba bean (Vicia faba L.) based on morphological traits and molecular markers, vol. 14, pp. 7587-7596, 2015.

Genetic diversity within Vicia faba L. is key to the ge­netic improvement of this important species. In this study, morphologi­cal traits and RAPD molecular markers were used to assess the levels of polymorphism across 12 Tunisian populations, three major and nine minor from different locations. Analysis of morphological traits indi­cated that the three major populations showed significant differences and the nine minor populations exhibited considerable variation for most traits. The grain yield of the Alia population could be increased by inoculation.

Genetic diversity analysis of tree peony germplasm using iPBS markers

Y. B. Duan, Guo, D. L., Guo, L. L., Wei, D. F., and Hou, X. G., Genetic diversity analysis of tree peony germplasm using iPBS markers, vol. 14, pp. 7556-7566, 2015.

We examined the genetic diversity of 10 wild species (populations) and 55 varieties of tree peony using inter-primer binding site (iPBS) markers. From a total of 36 iPBS primers, 16 were selected based on polymorphic amplification. The number of bands amplified by each primer ranged from 9 to 19, with an average of 12.88 bands per primer. The length of bands ranged from 100 to 2000 bp, concentrated at 200 to 1800 bp. Sixteen primers amplified 206 bands in total, of which 173 bands were polymorphic with a polymorphism ratio of 83.98%.

Subscribe to Genetic diversity