Genetic markers

Isolation and characterization of novel microsatellite markers for molecular genetic diversity in Siganus fuscescens

Y. F. Ning, Li, Z. B., Li, Q. H., Dai, G., Shangguan, J. B., Yuan, Y., and Huang, Y. S., “Isolation and characterization of novel microsatellite markers for molecular genetic diversity in Siganus fuscescens”, vol. 14, pp. 89-92, 2015.

The rabbitfish Siganus fuscescens is an economically valuable species that is widely distributed throughout the estuaries, intertidal, and offshore coasts of the Indo-Pacific and eastern Mediterranean. Ten novel microsatellite loci from the genome of S. fuscescens were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats. Polymorphisms in these 10 microsatellite markers were determined from 32 wild individuals.

Development of novel and polymorphic microsatellite DNA loci from Haliotis ovina

Z. B. Li, Tian, X. J., Shangguan, J. B., Cao, Y. Y., Zhang, G. L., and Chen, L. N., “Development of novel and polymorphic microsatellite DNA loci from Haliotis ovina”, vol. 14, pp. 525-528, 2015.

Twelve microsatellite loci were developed from Haliotis ovina by the magnetic bead hybridization method. Genetic variability was assessed using 30 individuals from 3 wild populations. The number of alleles per locus ranged from 2 to 5, and the polymorphism information content ranged from 0.1228 to 0.6542. Observed and expected heterozygosities ranged from 0.0000 to 0.7778 and 0.1288 to 0.6310, respectively.

Isolation of new polymorphic microsatellite markers from the marbled rockfish Sebastiscus marmoratus

H. W. Deng, Li, Z. B., Dai, G., Yuan, Y., Ning, Y. F., Shangguan, J. B., and Huang, Y. S., “Isolation of new polymorphic microsatellite markers from the marbled rockfish Sebastiscus marmoratus”, vol. 14, pp. 758-762, 2015.

The marbled rockfish, Sebastiscus marmoratus, is an important commercially near-shore fish that inhabits the beach rocky bottom from Japan to the South China Sea. Eleven polymorphic microsatellite loci were developed from S. marmoratus and were used to identify polymorphisms in 30 samples from a wild population. The allele locus number ranged from 2 to 7. Polymorphism data content ranged from 0.032 to 0.751. The observed and expected heterozygosity levels were 0.0333-0.9667 and 0.0328-0.7675, respectively.

Isolation and characterization of novel polymorphic microsatellite loci in Atrina vexillum Born (Pinnidae)

Y. S. Huang, Li, Q. H., Li, Z. B., Shangguan, J. B., Ning, Y. F., and Dai, G., “Isolation and characterization of novel polymorphic microsatellite loci in Atrina vexillum Born (Pinnidae)”, vol. 14, pp. 3541-3544, 2015.

The pen shell, Atrina vexillum Born, is an edible shellfish that is widely consumed in the Asia-Pacific region. In this study, 11 polymorphic microsatellite loci were isolated from A. vexillum, and 30 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 3 to 8. The polymorphism information content varied from 0.199 to 0.831. The observed and expected heterozygosities were 0.1000-0.8667 and 0.1244-0.8356, respectively.

Development and characterization of microsatellite loci in Megalonibea fusca

Z. B. Li, Li, Q. H., Ning, Y. F., Shangguan, J. B., Dai, G., Chen, L. N., Cao, Y. Y., and Chen, X. J., “Development and characterization of microsatellite loci in Megalonibea fusca”, vol. 14, pp. 4633-4636, 2015.

Megalonibea fusca is a commercially important large edible fish. In this study, the first set of 10 polymorphic microsatellite loci for M. fusca was developed and characterized. The number of alleles per locus ranged from two to five, with the observed and expected heterozygosities ranging from 0.0667 to 0.7667, and from 0.0644 to 0.5828, respectively. Most of the loci were in Hardy-Weinberg equilibrium (P > 0.05), except for two loci (Mf25 and Mf30) after a Bonferroni’s correction (P

Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)

Z. B. Li, Li, Q. H., Shangguan, J. B., Ning, Y. F., and Dai, G., “Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)”, vol. 14. pp. 6319-6322, 2015.

Lunella coronata granulata, from the family Turbini­dae, is an economically important species. The first set of 10 poly­morphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, respectively. The polymorphism information content varied from 0.305 to 0.559.

Cross-amplification and characterization of microsatellite loci in Acropora austera from the south-western Indian Ocean

P. H. Montoya-Maya, Macdonald, A. H. H., and Schleyer, M. H., “Cross-amplification and characterization of microsatellite loci in Acropora austera from the south-western Indian Ocean”, vol. 13, pp. 1244-1250, 2014.

Here, we report the successful cross-species amplification of previously published acroporid microsatellite markers in the coral Acropora austera from the south-western Indian Ocean. This fast-growing species is a major reef-building coral on South African reefs; however, it is the most damaged coral by scuba diving activity, and is known to be very susceptible to coral bleaching. Neither genetic information nor symbiont-free host tissue was available to develop novel microsatellite markers for this species.

Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)

X. J. Chen, Li, Z. B., Chen, L., Cao, Y. Y., and Li, Q. H., “Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)”, vol. 11, pp. 2884-2887, 2012.

The pen shell, Atrina pectinata, is a commercially important bivalve species, widely consumed in the Asian Pacific region. We identified 16 new microsatellite makers for A. pectinata using a modified fast isolation by AFLP of sequences containing repeat protocols; 27 individuals were collected from Xiamen to evaluate the degree of polymorphism. The number of polymorphic alleles per locus ranged from 2 to 11. The observed and expected heterozygosities were 0.050-0.913 and 0.049-0.869, respectively.

Characterization of new microsatellite markers of Siganus fuscescens (Siganidae)

Q. H. Li, Li, Z. B., Dai, G., Chen, X. J., Chen, L. N., Cao, Y. Y., Shangguan, J. B., and Ning, Y. F., “Characterization of new microsatellite markers of Siganus fuscescens (Siganidae)”, vol. 12, pp. 2751-2754, 2013.

Siganus fuscescens, which is a small commercially important marine fish, is wildly distributed in shallow waters throughout the tropical and subtropical Indo-Pacific and Eastern Mediterranean regions. It is part of a group known as rabbitfish. Fifteen new polymorphic microsatellite markers for S. fuscescens were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 2 to 12, and the polymorphism information content ranged from 0.210 to 0.849.

Genetic structure of Aedes aegypti populations determined using pairwise comparisons

deF. T. Patarro, Guirado, M. M., Ravazzi, L. M., and Bicudo, H. E. Mde C., “Genetic structure of Aedes aegypti populations determined using pairwise comparisons”, vol. 12, pp. 3775-3787, 2013.

The biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six primers. Four populations were from Brazil and one was from the USA. A total of 165 polymorphic DNA loci were generated.

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