Genetic structure

Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites

C. Wang, Zhang, S. J., Du, X. Y., Xu, Y. M., Huo, X. Y., Liao, L. F., and Chen, Z. W., “Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites”, vol. 14, pp. 14339-14347, 2015.

The grey hamster has been used in biomedical research for decades. However, effective molecular methods for evaluating the genetic structure of this species are lacking, which hinders its wider usage. In this study, we employed cross-amplification of microsatellite loci of species within the same genus by polymerase chain reaction. Loci screened included 107 from the Mongolian gerbil (MG) and 60 from the Chinese hamster (CH); of these, 15 polymorphic loci were identified for the grey hamster.

Development of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae

B. B. Cheng, Sun, Q. W., and Zheng, Y. Q., “Development of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae”, vol. 14, pp. 16018-16023, 2015.

Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross-amplification of the 9 loci was observed in 2 other varieties of T. wallichiana and 4 other Taxaceae species. These loci will be of value for studying population genetic structures and for genetic resource conservation in T.

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