geNorm

Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head

X. N. Wang, Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., Wang, J. C., Wang, X. N., Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., and Wang, J. C., Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head, vol. 15, p. -, 2016.

This study aimed to evaluate 12 genes (18S, GAPDH, B2M, ACTB, ALAS1, GUSB, HPRT1, PBGD, PPIA, PUM1, RPL29, and TBP) for their reliability and stability as reference sequences for real-time quantitative PCR (RT-qPCR) in bone marrow-derived mesenchymal stem cells (BMSCs) isolated from patients with avascular necrosis of the femoral head (ANFH). BMSCs were isolated from 20 ANFH patients divided into four groups according to etiology, and four donors with femoral neck fractures.

Selection and use of reference genes in mouse mammary glands

L. Q. Han, Yang, G. Y., Zhu, H. S., Wang, Y. Y., Wang, L. F., Guo, Y. J., Lu, W. F., Li, H. J., and Wang, Y. L., Selection and use of reference genes in mouse mammary glands, vol. 9, pp. 449-456, 2010.

Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software to eight commonly used reference genes to identify the most stable and optimal genes for the mouse mammary gland. Based on this analysis, HPRT, RPL and GAPDH are the most appropriate reference genes for data normalization.

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