Genotyping

Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle

M. M. Dias, Souza, F. R. P., Takada, L., Feitosa, F. L. B., Costa, R. B., Diaz, I. D. P. S., Cardoso, D. F., Tonussi, R. L., Baldi, F., Albuquerque, L. G., and Oliveira, H. N., “Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle”, vol. 14, pp. 234-243, 2015.

The objective of this study was to identify associations between known polymorphisms in genes related to adipose tissue and sexual precocity in Nellore cattle. A total of 1689 precocious and non-precocious heifers belonging to farms participating in Conexão Delta G breeding program were studied. SNPs from the Illumina High-Density Bovine SNP BeadChip were used. This chip contains 777,000 SNPs located within the region of the candidate genes at a distance of up to 5 kb, considering that linkage disequilibrium (LD) exists at this distance.

Identification of novel compound heterozygous RECQL4 mutations and prenatal diagnosis of Baller-Gerold syndrome: a case report

D. H. Cao, Mu, K., Liu, D. N., Sun, J. L., Bai, X. Z., Zhang, N., Qiu, G. B., and Ma, X. W., “Identification of novel compound heterozygous RECQL4 mutations and prenatal diagnosis of Baller-Gerold syndrome: a case report”, vol. 14. pp. 4757-4766, 2015.

Birth defects are structural and/or functional malforma­tions present at birth that cause physical or mental disability and are im­portant public health problems. Our study was aimed at genetic analysis and prenatal diagnosis of congenital anomalies to understand the cause of certain birth defects. Karyotypes and array-comparative genomic hy­bridization (aCGH) were performed on a pregnant woman, surrounding amniotic fluid, and her husband. A short-stature panel genetic test was conducted in accordance with the phenotype of the fetus.

Effect of CYP2C9*3 mutant variants on meloxicam pharmacokinetics in a healthy Chinese population

M. Zhang, Yang, Y., Zhao, G., Di, X., Xu, L., Jiang, N., Xu, J., and Xu, X., “Effect of CYP2C9*3 mutant variants on meloxicam pharmacokinetics in a healthy Chinese population”, vol. 13, pp. 831-837, 2014.

The aim of this study was to investigate the effect of the CYP2C9*3 (CYP2C9 1075 A>C) polymorphism on meloxicam pharmacokinetics in a Chinese population. Twenty-four healthy volunteers were enrolled in this study. The pyrosequencing technique was used to identify polymorphisms of CYP2C9. The concentration of meloxicam in plasma was determined by a high-performance liquid chromatography assay with mass spectrographic analysis. The Drug and Statistics Software (DAS, version 2.0) was used for curve fitting and calculations of pharmacokinetic parameters.

Cross-amplification and characterization of microsatellite loci in Acropora austera from the south-western Indian Ocean

P. H. Montoya-Maya, Macdonald, A. H. H., and Schleyer, M. H., “Cross-amplification and characterization of microsatellite loci in Acropora austera from the south-western Indian Ocean”, vol. 13, pp. 1244-1250, 2014.

Here, we report the successful cross-species amplification of previously published acroporid microsatellite markers in the coral Acropora austera from the south-western Indian Ocean. This fast-growing species is a major reef-building coral on South African reefs; however, it is the most damaged coral by scuba diving activity, and is known to be very susceptible to coral bleaching. Neither genetic information nor symbiont-free host tissue was available to develop novel microsatellite markers for this species.

Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep

M. Yang, Yue, Y. J., Guo, T. T., Han, J. L., Liu, J. B., Guo, J., Sun, X. P., Feng, R. L., Wu, Y. Y., Wang, C. F., Wang, L. P., and Yang, B. H., “Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep”, vol. 13, pp. 2645-2653, 2014.

Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity and accuracy. In this report, we assessed the latest of the non-gel-based methods, high-resolution melting (HRM) curve analysis.

Gene polymorphisms associated with susceptibility to coronary artery disease in Han Chinese people

Y. H. Liu, Zhou, Y. W., Yang, J. A., Tu, Z. G., Ji, S. Y., Huang, Z. Y., and Zhou, Z. J., “Gene polymorphisms associated with susceptibility to coronary artery disease in Han Chinese people”, vol. 13, pp. 2619-2627, 2014.

This study investigated 5 single nucleotide polymorphism (SNP) haplotypes in susceptibility genes for coronary artery disease (CAD) and the putative involvement of these SNPs in CAD in the Chi­nese Han population. From March 2008 to June 2009, we selected 119 CAD patients and 115 subjects not related to the CAD of Chinese Han or­igin as controls. The SNP genotypes were performed by multiplex SNaP­shot technology. The HNRPUL1 gene rs11881940T and GATA2 gene rs3803T loci were highly correlated with CAD (P

Comparison of a retrotransposon-based marker with microsatellite markers for discriminating accessions of Vitis vinifera

G. C. Sant’Ana, Ferreira, J. L., Rocha, H. S., Borém, A., Pasqual, M., and Cançado, G. M. A., “Comparison of a retrotransposon-based marker with microsatellite markers for discriminating accessions of Vitis vinifera”, vol. 11, pp. 1507-1525, 2012.

Identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. Recently, new types of molecular markers have been developed, such as retrotransposon-based markers. Because of their multilocus pattern, retrotransposon-based markers might be able to differentiate grapevine accessions with just one pair of primers.

Ovine prion protein genotype frequencies in northwestern China

C. - L. Zhao, Wu, R., Liu, L., Li, F. - D., Zhang, X. - L., Wang, C., Wang, F., Diao, X. - L., Guan, H. - W., Wang, X., and Zhou, L., “Ovine prion protein genotype frequencies in northwestern China”, vol. 11, pp. 1671-1681, 2012.

Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep breeds. We analyzed 432 sheep of five different breeds from farms in northwestern China, using PCR-single-strand conformational polymorphism analysis (PCR-SSCP); the corresponding haplotypes of different PRNP alleles were cloned.

Allelic database and accession divergence of a Brazilian mango collection based on microsatellite markers

I. C. Ndos Santos Ribeiro, Neto, F. P. Lima, and Santos, C. A. F., “Allelic database and accession divergence of a Brazilian mango collection based on microsatellite markers”, vol. 11, pp. 4564-4574, 2012.

Allelic patterns and genetic distances were examined in a collection of 103 foreign and Brazilian mango (Mangifera indica) accessions in order to develop a reference database to support cultivar protection and breeding programs. An UPGMA dendrogram was generated using Jaccard’s coefficients from a distance matrix based on 50 alleles of 12 microsatellite loci. The base pair number was estimated by the method of inverse mobility. The cophenetic correlation was 0.8. The accessions had a coefficient of similarity from 30 to 100%, which reflects high genetic variability.

Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA

E. Yörük and Albayrak, G., “Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA”, vol. 12, pp. 1360-1372, 2013.

Five Fusarium graminearum and 12 F. culmorum isolates, primarily pathogenic species of Fusarium head blight, were obtained from naturally infected wheat from various agro-ecological regions of Turkey. Genotyping of the isolates was carried out using random-amplified polymorphic DNA (RAPD). Sixty-five 10-mer oligonucleotide primers were used to amplify the RAPD markers. Among them, 50 primers produced strong and reproducible DNA amplicons. The remaining primers generated either insufficient or no amplification patterns.

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