Giant panda

Molecular cloning, overexpression, purification, and sequence analysis of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide

L. Fu, Hou, Y. L., Ding, X., Du, Y. J., Zhu, H. Q., Zhang, N., Hou, W. R., Fu, L., Hou, Y. L., Ding, X., Du, Y. J., Zhu, H. Q., Zhang, N., and Hou, W. R., “Molecular cloning, overexpression, purification, and sequence analysis of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide”, vol. 15, p. -, 2016.

The complementary DNA (cDNA) of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide (FTL) gene was successfully cloned using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing FTL cDNA and overexpressed it in Escherichia coli using pET28a plasmids. The expressed protein was then purified by nickel chelate affinity chromatography. The cloned cDNA fragment was 580 bp long and contained an open reading frame of 525 bp.

Identification of conserved microRNAs in peripheral blood from giant panda: expression of mammary gland-related microRNAs during late pregnancy and early lactation

C. D. Wang, Long, K., Jin, L., Huang, S., Li, D. H., Ma, X. P., Wei, M., Gu, Y., Ma, J. D., and Zhang, H., “Identification of conserved microRNAs in peripheral blood from giant panda: expression of mammary gland-related microRNAs during late pregnancy and early lactation”, vol. 14, pp. 14216-14228, 2015.

The giant panda (Ailuropoda melanoleuca) is one of the world’s most endangered mammals, and it has evolved several unusual biological and behavioral traits. During puberty, pregnancy, lactation, and involution, the mammary gland undergoes profound morphological and functional changes. A large number of microRNAs (miRNAs) have been identified to be involved in mammary gland development and lactation. In this study, we identified 202 conserved mature miRNAs, corresponding to 147 pre-miRNAs, in giant panda peripheral blood using a small RNA-sequencing approach.

Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca)

B. Song, Hou, Y. L., Ding, X., Wang, T., Wang, F., Zhong, J. C., Xu, T., Zhong, J., Hou, W. R., and Shuai, S. R., “Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca)”, vol. 13, pp. 992-1004, 2014.

Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction (RT-PCR) technology and touchdown-PCR.

Molecular cloning, characterization, and ioactivity analysis of interleukin 18 in giant panda (Ailuropoda melanoleuca)

Y. Yan, Wang, Q., Niu, L. L., Deng, J. B., Yu, J. Q., Zhang, Y. Z., Wang, J. X., Yin, M. M., and Tan, X. M., “Molecular cloning, characterization, and ioactivity analysis of interleukin 18 in giant panda (Ailuropoda melanoleuca)”, vol. 13, pp. 9687-9700, 2014.

Interleukin 18 (IL-18), as a member of IL-1 superfamily, is an important pleiotropic cytokine that modulates Th1 immune responses. In this report, we cloned and identified a homolog of IL-18 in giant panda (Ailuropoda melanoleuca) (designated as AmIL-18) from peripheral blood mononuclear cells stimulated with lipopolysaccharide. The open readin g frame of AmIL-18 cDNA is 579 bp encoding a deduced protein of 192 amino acids. AmIL-18 gDNA fragments contained 5 exons and 4 introns.

cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1

W. - R. Hou, Hou, Y. - L., Ding, X., and Wang, T., “cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1”, vol. 11, pp. 3164-3174, 2012.

The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp encoding 136 amino acids; the length of the genomic sequence is of 1838 bp, containing three exons and two introns.

Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein

Y. Yi, Nian, Y. - Y., Ji, H. - W., Zhang, H., Zhu, L., and Xu, Z. - W., “Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein”, vol. 12, pp. 1987-1995, 2013.

To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriacetic acid column. The final production of IL-6/IL-2 reached 6 mg/L in soluble form, and the purified final product was >96% pure.

Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)

Y. L. Hou, Ding, X., Hou, W., Song, B., Wang, T., Wang, F., Li, J., Zhong, J., Xu, T., Ma, B. X., Zhu, H. Q., Li, J. H., and Zhong, J. C., “Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)”, vol. 12, pp. 4735-4750, 2013.

The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing RPL24 complementary DNA and overexpressed it in Escherichia coli using pET28a plasmids.

Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression

G. - F. Wu, Hou, Y. - L., Hou, W. - R., Song, Y., and Zhang, T., “Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression”, vol. 9, pp. 2004-2015, 2010.

RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The length of the cloned cDNA fragment was 492 bp; it contained an open-reading frame of 456 bp, encoding 151 amino acids. The length of the genomic sequence is 3421 bp; it contains four exons and three introns.

cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)

Y. Song, Hou, Y. - L., Hou, W. - R., Wu, G. - F., and Zhang, T., “cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)”, vol. 10, pp. 121-132, 2011.

The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherichia coli BL21. We compared the nucleotide sequences of the coding region and the amino acid sequences with those of seven other mammalian species retrieved from GenBank.

Subscribe to Giant panda