As a “living fossil” that is used to understand the evolutionary history of seed plants, Ginkgo biloba is a well-known multipurpose tree with edible seeds, medicinal properties, and ornamental value, but little is known about its genetic diversity. Microsatellite, or simple sequence repeat (SSR), markers have proven to be powerful tools for genetic studies of plants. In this study, we isolated 30 novel polymorphic microsatellite loci in G. biloba using 454 pyrosequencing. The characteristics of these loci were tested with 48 cultivars.
Ginkgo biloba is considered to be a living fossil that can be used to understand the ancient evolutionary history of gymnosperms, but little attention has been given to the study of its population genetics, molecular phylogeography, and genetic resources assessment. Chloroplast simple sequence repeat (cpSSR) markers are powerful tools for genetic studies of plants. In this study, a total of 30 perfect cpSSRs of Ginkgo were identified and characterized, including di-, tri, tetra-, penta-, and hexanucleotide repeats.