GluR-B minigene

Dephosphorylation of NSSR1 regulates alternative splicing of the GluR-B minigene

W. Zhang, Li, L. L., Chen, X. H., and Peng, Z. Y., Dephosphorylation of NSSR1 regulates alternative splicing of the GluR-B minigene, vol. 13, pp. 1753-1763, 2014.

Neural salient serine/arginine-rich protein 1 (NSSR1, alternatively SRp38) is an important splicing factor that can repress pre-mRNA alternative splicing in cells during heat shock and mitosis. We show here that NSSR1 protein is dephosphorylated when cells are heat shocked or incubated with kinase inhibitor K252a. Both heat shock and K252a treatment increase the truncated splicing isoform of the GluR-B minigene pre-mRNA. We also investigated the roles of the RRM motif and three RS domains of NSSR1 in in vivo pre-mRNA splicing.

Subscribe to GluR-B minigene