Goose

Characterization of HSP70 and its expression in tissue: correlation with physiological and immune indices in goose (Anser cygnoides) serum

W. W. Zhang, Xiao, X., Gan, J. K., Zhang, X. Q., Kong, L. N., and Luo, Q. B., Characterization of HSP70 and its expression in tissue: correlation with physiological and immune indices in goose (Anser cygnoides) serum, vol. 14, pp. 12288-12298, 2015.

We cloned the goose heat shock protein 70 gene (HSP70), to determine its sequence variation and elucidate its mRNA expression. We designed primers to amplify the entire goose HSP70 sequence. We used 10 commercial Wuzong goslings in a heat-stress experiment. We collected tissue samples for RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR). We analyzed the variation in expression of goose HSP70 before and after heat stress. We constructed a DNA pool from six different species, for single nucleotide polymorphism (SNP) screening.

Avian sarcoma and leukosis virus gag gene in the Anser anser domesticus genome

F. Zhu, Jie, H., Lian, L., Qu, L. J., Hou, Z. C., Zheng, J. X., Chen, S. Y., Yang, N., and Liu, Y. P., Avian sarcoma and leukosis virus gag gene in the Anser anser domesticus genome, vol. 14, pp. 14379-14386, 2015.

Endogenous retroviruses are regarded as ideal genetic markers for evolutionary analyses. Birds were some of the initial vertebrates found to contain endogenous retroviruses. However, few studies have investigated the presence and distribution of endogenous retroviruses in goose. In this study, we detected the avian sarcoma and leukosis virus gag gene in the genomic DNA of 8 Chinese native breeds using polymerase chain reaction method. The results indicated that a 1.2-kb avian sarcoma and leukosis virus gag sequence was integrated into all 8 goose breeds.

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