Grape

Comparative analysis of MIR168 promoters in three plant species

D. Qu, Yan, F., Li, M. A., Varotto, C., Zhao, Z. Y., Qu, D., Yan, F., Li, M. A., Varotto, C., and Zhao, Z. Y., “Comparative analysis of MIR168 promoters in three plant species”, vol. 15, p. -, 2016.

MicroRNAs (miRNAs) play important roles in the regulation of gene expression by post-transcriptionally targeting mRNAs for cleavage or translational repression. miR168 is a key miRNA because it regulates the expression of the slicer protein ARGONAUTE1 (AGO1), which catalyzes mRNA cleavage. Interestingly, plant miR168s are highly evolutionarily conserved; however, it is unclear whether MIR168 promoter elements and expression patterns are also conserved. Here, we isolated MIR168 promoters from monocot rice and dicot grape genomes.

In vitro embryo rescue culture of F1 progenies from crosses between different ploidy grapes

W. Ji, Li, G. R., Luo, Y. X., Ma, X. H., Wang, M., and Ren, R., “In vitro embryo rescue culture of F1 progenies from crosses between different ploidy grapes”, vol. 14, pp. 18616-18622, 2015.

Crossing different ploidy grapes is an effective way to obtain new seedless cultivars. Although embryo rescue has been extensively applied in breeding seedless and triploid grapes, only a few improved cultivars have been developed. Based on preliminary studies, we set five crosses between tetraploid and diploid grape varieties to obtain new hybrid triploid germplasms. Additionally, we compared two different methods of performing in vitro embryo rescue and sowing in the development of hybrid triploid grape plants.