Hepatocytes

Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library

Y. H. Gao, Wang, K. F., Zhang, S., Fan, Y. N., Guan, W. J., and Ma, Y. H., Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library, vol. 14, pp. 9764-9771, 2015.

Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expression vector (pEGFP-N3-L-FABP) was constructed in vitro. This vector was transfected into hepatocytes to test its function.

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