High-GC primer

An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers

C. L. Wei, Cheng, J. L., Khan, M. A., Yang, L. Q., Imani, S., Chen, H. C., Fu, J. J., Wei, C. L., Cheng, J. L., Khan, M. A., Yang, L. Q., Imani, S., Chen, H. C., and Fu, J. J., An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers, vol. 15, p. -, 2016.

Random amplified polymorphic DNA (RAPD) is a widely used molecular marker technique. As traditional RAPD has poor reproducibility and productivity, we previously developed an improved RAPD method (termed RAMP-PCR), which increased the reproducibility, number of bands, and efficiency of studies on polymorphism. To further develop the efficiency of this method, we used high-GC content primers for improved RAMP-PCR with DNA samples from Lonicera japonica.

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