High-quality protocol

A high-throughput, high-quality plant genomic DNA extraction protocol

H. Li, Li, J., Cong, X. H., Duan, Y. B., Li, L., Wei, P. C., Lu, X. Z., and Yang, J. B., A high-throughput, high-quality plant genomic DNA extraction protocol, vol. 12, pp. 4526-4539, 2013.

The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block, our protocol provides high throughput. Without the need for phenol-chloroform and liquid nitrogen or dry ice, our protocol is safer and more cost-efficient than traditional DNA extraction methods.

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