Hormone

Roles of oxidative DNA damage of bone marrow hematopoietic cells in steroid-induced avascular necrosis of femoral head

Z. Q. Zhao, Bai, R., Liu, W. L., Feng, W., Zhao, A. Q., Wang, Y., Wang, W. X., Sun, L., Wu, L. S., Cui, S. X., Zhao, Z. Q., Bai, R., Liu, W. L., Feng, W., Zhao, A. Q., Wang, Y., Wang, W. X., Sun, L., Wu, L. S., Cui, S. X., Zhao, Z. Q., Bai, R., Liu, W. L., Feng, W., Zhao, A. Q., Wang, Y., Wang, W. X., Sun, L., Wu, L. S., and Cui, S. X., Roles of oxidative DNA damage of bone marrow hematopoietic cells in steroid-induced avascular necrosis of femoral head, vol. 15, p. -, 2016.

An animal model of steroid-induced avascular necrosis of femoral head (SANFH) was established to investigate the role of oxidative DNA damage of bone marrow hematopoietic cells in SANFH. Forty-five-month-old Japanese white rabbits (male or female, 2.5 ± 0.5 kg) were randomly divided into groups A (methylprednisolone + Escherichia coli endotoxin), B (methylprednisolone alone), C (E. coli endotoxin alone), and D (blank control). The animals were sacrificed two and four weeks after administration of the last dose (N = 5 each group and each time).

Roles of osteocyte apoptosis in steroid-induced avascular necrosis of the femoral head

R. Bai, Feng, W., Liu, W. L., Zhao, Z. H. Q., Zhao, A. Q., Wang, Y., Wang, W. X., Sun, L., Wu, L. S. H., Cui, S. H. X., Bai, R., Feng, W., Liu, W. L., Zhao, Z. H. Q., Zhao, A. Q., Wang, Y., Wang, W. X., Sun, L., Wu, L. S. H., Cui, S. H. X., Bai, R., Feng, W., Liu, W. L., Zhao, Z. H. Q., Zhao, A. Q., Wang, Y., Wang, W. X., Sun, L., Wu, L. S. H., and Cui, S. H. X., Roles of osteocyte apoptosis in steroid-induced avascular necrosis of the femoral head, vol. 15, p. -, 2016.

An animal model of steroid-induced avascular necrosis of the femoral head (SANFH) was established to investigate the roles of osteocyte apoptosis in this process. Forty five-month-old male and female Japanese white rabbits were randomly divided into groups A (hormone + endotoxin), B (hormone alone), C (endotoxin alone), and D (blank control). Animals were sacrificed two and four weeks following the final treatment (N = 5 for each group at each time point). Bilateral femoral heads were fixed and decalcified, and empty lacunae were counted by hematoxylin staining.

Molecular cloning and characterization of GbMECT and GbMECP gene promoters from Ginkgo biloba

S. Y. Cheng, Li, L. L., Yuan, H. H., Xu, F., and Cheng, H., Molecular cloning and characterization of GbMECT and GbMECP gene promoters from Ginkgo biloba, vol. 14, pp. 15112-15122, 2015.

Ginkgolides are key pharmaceutical components in Ginkgo biloba. Using the cDNA sequence of the MECP and MECT genes to design primers, we obtained the promoters of these genes from Ginkgo genomic DNA using the genome walking method. The two promoters were 744 and 982 bp in length, respectively. The cis-elements of the GbMECPs and GbMECT promoters were predicted and analyzed using the plant cis-acting regulatory element database. We found major cis-elements in the sequence of the GbMECT and GbMECPs promoters.

Effect of dehydroepiandrosterone treatment on hormone levels and antioxidant parameters in aged rats

F. J. Yin, Kang, J., Han, N. N., and Ma, H. T., Effect of dehydroepiandrosterone treatment on hormone levels and antioxidant parameters in aged rats, vol. 14, pp. 11300-11311, 2015.

The aim of the current study was to evaluate the effect of chronic dehydroepiandrosterone (DHEA) administration on steroid hormones and antioxidant parameters in aged rats. To this end, three groups of Sprague-Dawley rats were compared: young (3 months of age) untreated; aged (19 months old) untreated; and aged rats treated with 20 mg/kg DHEA for 8 weeks. Major organs of aged rats in the untreated group demonstrated physiological atrophy, compared to those of young rats; this effect appeared to have been partially reversed by DHEA treatment.

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