Hsp

mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

X. N. Bao, Mu, C. K., Zhang, C., Wang, Y. F., Song, W. W., Li, R. H., and Wang, C. L., mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress, vol. 13, pp. 6837-6847, 2014.

Challenged by the low salinity, 4 parts per thousand (4 ppt), for 72h, the survivals of swimming crabs (Portunus trituberculatus) were collected as the screened group (SG, tolerant to low salinity). Aiming at identifying the mechanism of low salinity tolerance, quantitative real-time PCR was employed to investigate the expression profiles of 4 HSP genes (HSP60, HSP70, HSP90-1, HSP90-2) in the hepatopancreas of wild (WG) and screened (SG) groups of P. trituberculatus exposed to low salinity (4 ppt).

Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum

I. B. Cordeiro, Castro, D. P., Nogueira, P. P. O., Angelo, P. C. S., Nogueira, P. A., Gonçalves, J. F. C., Pereira, A. M. R. F., Garcia, J. S., Souza, G. H. M. F., Arruda, M. A. Z., Eberlin, M. N., Astolfi-Filho, S., Andrade, E. V., and López-Lozano, J. L., Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum, vol. 12, pp. 5057-5071, 2013.

Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 380 in each assay.

Localization of HSP single-copy genes by inexpensive, permanent non-fluorescent in situ hybridization on meiotic chromosomes of the grasshopper Schistocerca pallens (Acrididae)

T. T. Rieger, Oliveira-Silva, S. V., Pachêco, I. A., Chagas, B. S., and Santos, J. F., Localization of HSP single-copy genes by inexpensive, permanent non-fluorescent in situ hybridization on meiotic chromosomes of the grasshopper Schistocerca pallens (Acrididae), vol. 6, pp. 643-649, 2007.

There have been many studies on Schistocerca gregaria and Locusta migratoria, which are important grasshopper pests in many parts of the world. However, the main pest grasshopper species in Brazil, S. pallens, Rhammatocerus schistocercoides and Stiphra robusta, are very poorly characterized genetically. We adapted a permanent in situ hybridization method to extend the genetic characterization of S.

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