HSP70

Analysis of genetic diversity of the heat shock protein 70 gene on the basis of abundant sequence polymorphisms in chicken breeds

J. K. Gan, Jiang, L. Y., Kong, L. N., Zhang, X. Q., and Luo, Q. B., Analysis of genetic diversity of the heat shock protein 70 gene on the basis of abundant sequence polymorphisms in chicken breeds, vol. 14, pp. 1538-1545, 2015.

This study was designed to detect the sequence variation of the chicken heat shock protein 70 (HSP70) gene. A total of 102 individuals from 8 native Chinese breeds together with Dwarf White Chicken and Red Junglefowl were used to detect sequence variations. The coding regions of the chicken HSP70 gene from 102 individuals were cloned and sequenced. Thirty-six variations were identified, which included 34 single nucleotide polymorphisms and 2 indel mutations. Fifty-seven haplotypes were observed, of which, 43 were breed-specific and 14 were shared.

Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo

H. B. Chen, Zhang, X. C., Cheng, Y. F., Abdelnasir, A., Tang, S., Kemper, N., Hartung, J., and Bao, E. D., Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo, vol. 14, pp. 1994-2005, 2015.

To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro.

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

J. K. Gan, Zhang, D. X., He, D. L., Zhang, X. Q., Chen, Z. Y., and Luo, Q. B., Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress, vol. 12, pp. 809-819, 2013.

DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dropping. Hypomethylation of the HSP70 promoter occurred in all of the groups studied, but the difference between groups within tissue type was not significant.

Molecular cloning of HSP70 in Mycoplasma ovipneumoniae and comparison with that of other mycoplasmas

M. Li, Ma, C. J., Liu, X. M., Zhao, D., Xu, Q. C., and Wang, Y. J., Molecular cloning of HSP70 in Mycoplasma ovipneumoniae and comparison with that of other mycoplasmas, vol. 10, pp. 834-848, 2011.

Mycoplasma ovipneumoniae, a bacterial species that specifically affects ovine and goat, is the cause of ovine infectious pleuropneumonia. We cloned, sequenced and analyzed heat shock protein 70 (HSP70) (dnaK) gene of M. ovipneumoniae. The full length open reading frame of the M. ovipneumoniae HSP70 gene consists of 1812 nucleotides, with a G+C content of 34.16%, encoding 604 amino acids.

Genetic evaluation of the HSP70 protein in the japanese quail (Coturnix japonica)

H. C. T. Gaviol, Gasparino, E., Prioli, A. J., and Soares, M. A. M., Genetic evaluation of the HSP70 protein in the japanese quail (Coturnix japonica), vol. 7, pp. 133-139, 2008.

Heat stress is one of the main problems in modern aviculture, since it affects birds especially in the final phase of rearing, causing bird mortality and economic losses to the aviculturist. The quail, as most birds, has difficulties in dissipating heat. However, little is known about the mechanism that controls the responses of the organism to stressor agents. Therefore, the study of heat shock proteins (HSPs) in these birds is important. A 960-bp portion of HSP70 was amplified using oligonucleotide primers specific for chickens.

Subscribe to HSP70