Hydrolytic enzymes

High yield of functional metagenomic library from mangroves constructed in fosmid vector

A. C. S. Gonçalves, Santos, A. C. Fdos, Santos, T. Fdos, Pessoa, T. B. A., Dias, J. C. T., and Rezende, R. P., High yield of functional metagenomic library from mangroves constructed in fosmid vector, vol. 14, pp. 11841-11847, 2015.

In the present study, metagenomic technique and fosmid vectors were used to construct a library of clones for exploring the biotechnological potential of mangrove soils by isolation of functional genes encoding hydrolytic enzymes. The library was built with genomic DNA from the soil samples of mangrove sediments and the functional screening of 1824 clones (~64 Mbp) was performed to detect the hydrolytic activity specific for cellulases, amylases (at acidic, neutral and basic pH), lipases/esterases, proteases, and nitrilases.

Enzymatic differences between the endophyte Guignardia mangiferae (Botryosphaeriaceae) and the citrus pathogen G. citricarpa

A. S. Romão, Spósito, M. B., Andreote, F. D., Azevedo, J. L., and Araújo, W. L., Enzymatic differences between the endophyte Guignardia mangiferae (Botryosphaeriaceae) and the citrus pathogen G. citricarpa, vol. 10, pp. 243-252, 2011.

The endophyte Guignardia mangiferae is closely related to G. citricarpa, the causal agent of citrus black spot; for many years these species had been confused with each other. The development of molecular analytical methods has allowed differentiation of the pathogen G. citricarpa from the endophyte G. mangiferae, but the physiological traits associated with pathogenicity were not described. We examined genetic and enzymatic characteristics of Guignardia spp strains; G.

Hydrolytic enzymes in Paracoccidioides brasiliensis - ecological aspects

B. Benoliel, Arraes, F. B. M., Reis, V. Castelo- Br, de Siqueira, S. J. L., Parachin, N. S., and Torres, F. A. G., Hydrolytic enzymes in Paracoccidioides brasiliensis - ecological aspects, vol. 4, pp. 450-461, 2005.

Paracoccidioides brasiliensis is a thermally dimorphic fungus that causes paracoccidioidomycosis. The yeast form of this pathogen is found in the animal host whereas the mycelial form is recovered from living and non-living organic material. The sole carbon source available in these habitats is represented by polysaccharides from the plant cell wall. Hydrolytic enzymes are necessary to convert these polymers into simple sugars for fungal metabolism. We report on the presence of ortholog genes of hydrolytic enzymes identified in the P.

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