DNA barcoding of arid wild plants using rbcL gene sequences

S. O. Bafeel, Arif, I. A., Bakir, M. A., Homaidan, A. A. Al, Farhan, A. H. Al, and Khan, H. A., DNA barcoding of arid wild plants using rbcL gene sequences, vol. 11, pp. 1934-1941, 2012.

DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. We therefore tested the potential of the rbcL marker for the identification of wild plants belonging to diverse families of arid regions.

Isolation and identification of bovine primary hepatocytes

Q. D. Jiang, Li, H. P., Liu, F. J., Wang, X. J., Guo, Y. J., Wang, L. F., Lu, W. F., Li, H. J., Li, X. P., and Yang, G. Y., Isolation and identification of bovine primary hepatocytes, vol. 12, pp. 5186-5194, 2013.

The liver is a unique organ that is endowed with a plethora of specialized functions. Most of its functional traits are controlled by hepatocytes. Primary hepatocytes have been used widely in in vitro models to understand the biological processes occurring in the liver. There are a number of methods used to separate hepatocytes, but the cell activity and purity are much lower in this condition. On the basis of previous research, in this study, the two-step collagenase perfusion technique was used for isolating hepatocytes.

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