Improved cetyltrimethylammonium bromide method

Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.

G. Q. Wen, Li, J., Liu, X. H., Zhang, Y. S., and Wen, S. S., Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb., vol. 13, pp. 1339-1347, 2014.

Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (RAPD) reaction system by design of the L16 (44) orthogonal diagram.

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