Ink staining

Diagnostic value of cytological and microbiological methods in cryptococcal meningitis

M. Zhang, Li, J. C., Lin, H., Zhang, W., Lin, M., Wu, L., Liu, W., Mu, J. S., Ye, J. X., and Cui, X. P., Diagnostic value of cytological and microbiological methods in cryptococcal meningitis, vol. 13, pp. 9253-9261, 2014.

The aim of this study was to investigate diagnostic methods for cryptococcal meningitis (CM). A retrospective analysis was conducted for 31 patients with CM confirmed by etiologic detection of cerebrospinal fluid in our hospital in the past 5 years. Nineteen cases in 31 patients were confirmed with CM in the first diagnosis, with a misdiagnosis rate of 38.7%. The positive rates of cryptococcus detection in cerebrospinal fluid with May-Grünwald-Giemsa (MGG)-, ink-, and Alcian blue-staining methods were 86.9, 70.9, and 80.6%, respectively.

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