Innate immunity

Sequence characterization and phylogenetic analysis of toll-like receptor (TLR) 4 gene in the Tibetan macaque (Macaca thibetana)

Q. X. Dai, Yao, Y. F., Qi, Z. C., Huang, Y., Ni, Q. Y., Zhang, M. W., and Xu, H. L., “Sequence characterization and phylogenetic analysis of toll-like receptor (TLR) 4 gene in the Tibetan macaque (Macaca thibetana)”, vol. 14, pp. 1875-1886, 2015.

In this study, the complete coding region sequence of an innate immune-related TLR4 gene was obtained from the Tibetan macaque (Macaca thibetana) genome via PCR and direct sequencing. The sequence had a total length of 2481 bp, contained 3 complete exons, and encoded 826 amino acids (AAs); its isoelectric point was 5.703, and the molecular weight was 94.72 kDa. The high structure prediction showed that the protein was comprised of one extracellular region, one transmembrane region, and one intracellular region.

Characterization and expression analysis of Toll-like receptor 3 cDNA from Atlantic salmon (Salmo salar)

R. Vidal, González, R., and Gil, F., “Characterization and expression analysis of Toll-like receptor 3 cDNA from Atlantic salmon (Salmo salar)”, vol. 14, pp. 6073-6083, 2015.

Innate pathway activation is fundamental for early anti-viral defense in fish, but currently there is insufficient understanding of how salmonid fish identify viral molecules and activate these pathways. The Toll-like receptor (TLR) is believed to play a crucial role in host defense of pathogenic microbes in the innate immune system. In the present study, the full-length cDNA of Salmo salar TLR3 (ssTLR3) was cloned. The ssTLR3 cDNA sequence was 6071 bp long, containing an open reading frame of 2754 bp and encoding 971 amino acids.

Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge

Q. Z. Yang, Yang, Z. J., Zhang, Y., Li, X. L., and Zhang, W., “Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge”, vol. 13, pp. 3885-3894, 2014.

A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H, D, and S). Similarity analysis shows that Es-CLSP shares high identity with CLSPs from the fiddler crab Uca pugilator. Es-CLSP mRNA expression in E.

Evolutionary analysis of the short-type peptidoglycan-recognition protein gene (PGLYRP1) in primates

W. Liu, Yao, Y. F., Zhou, L., Ni, Q. Y., and Xu, H. L., “Evolutionary analysis of the short-type peptidoglycan-recognition protein gene (PGLYRP1) in primates”, vol. 12, pp. 453-462, 2013.

Short-type peptidoglycan (PGN)-recognition protein 1 (PGLYRP1), an innate immunity protein that directly breaks down the structure of microbial cell wall PGNs, plays an important role both in antibacterial defenses and several inflammatory diseases.

Comparative phenotypic profile of subpopulations of peripheral blood leukocytes in European (Bos taurus taurus) and Zebu cattle (Bos taurus indicus)

A. A. Macêdo, Marciano, A. P. V., Rocha, L. M., Alves-Júnior, J. R. F., Faria, A. M. C., Bittar, J. F. F., Araújo, M. S. S., Santos, R. L., and Martins-Filho, O. A., “Comparative phenotypic profile of subpopulations of peripheral blood leukocytes in European (Bos taurus taurus) and Zebu cattle (Bos taurus indicus)”, vol. 12, pp. 6838-6849, 2013.

Differences in cellular and humoral immunity in Zebu (Bos taurus indicus) and European (B. taurus taurus) cattle breeds, which may be related to differences in resistance or susceptibility to infectious or parasitic diseases, are largely unknown.

Association of TIRAP (MAL) gene polymorhisms with susceptibility to tuberculosis in a Chinese population

Y. X. Zhang, Xue, Y., Zhao, M. Y., Wang, H. J., Li, J. C., Liu, J. Y., Li, F. J., and Zhou, J. M., “Association of TIRAP (MAL) gene polymorhisms with susceptibility to tuberculosis in a Chinese population”, vol. 10, pp. 7-15, 2011.

Toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP; also known as MAL) is an essential adaptor molecule in Toll-like receptor signaling, involved in activating the innate immune response during infection. Genetic variations in the TIRAP gene may influence human susceptibility to infectious disease. To date, in the Chinese population, a possible predisposition of TIRAP gene variants to tuberculosis has not been reported.

cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata

D. C. Zhang, Hu, Y. T., Guo, H. Y., Cui, S. G., Su, T. F., and Jiang, S. G., “cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata”, vol. 10, pp. 1963-1974, 2011.

Galectins can recognize and specifically bind to β-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. We cloned the cDNA of a tandem-repeat galectin from the pearl oyster Pinctada fucata (designated as PoGal2).

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