Interleukin-6/interleukin-2

Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein

Y. Yi, Nian, Y. - Y., Ji, H. - W., Zhang, H., Zhu, L., and Xu, Z. - W., Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein, vol. 12, pp. 1987-1995, 2013.

To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriacetic acid column. The final production of IL-6/IL-2 reached 6 mg/L in soluble form, and the purified final product was >96% pure.

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