Internal ribosome entry site

Construction and identification of pIRES2-VEGF165-NT-3 bicistronic eukaryotic expression vector

B. N. Li, Li, W. D., Feng, H. G., Lin, J. T., and Yuan, Z. Q., Construction and identification of pIRES2-VEGF165-NT-3 bicistronic eukaryotic expression vector, vol. 13, pp. 4704-4715, 2014.

We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-VEGF165-NT-3. The neurotrophin-3 (NT-3) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NT-3 cDNA fragment was cloned into the pIRES2-VEGF165-EGFP vector in place of enhanced green fluorescent protein (EGFP) to create the plasmid pIRES2-VEGF165-NT-3.

Construction and identification of pIRES2-LIF-NT-3 bicistronic eukaryotic expression vector

B. N. Li, Li, W. D., Lin, J. T., Feng, H. G., and Yuan, Z. Q., Construction and identification of pIRES2-LIF-NT-3 bicistronic eukaryotic expression vector, vol. 13, pp. 4691-4703, 2014.

We used a simple and efficient method to construct a bicistronic eukaryotic expression vector pIRES2-LIF-NT-3. The leukemia inhibitory factor (LIF) and neurotrophin-3 (NT-3) genes were obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The LIF cDNA fragment was inserted into the multiple cloning sites of a vector containing internal ribosome entry site and enhanced green fluorescent protein (EGFP) (pIRES2-EGFP) to generate the bicistronic eukaryotic expression plasmid pIRES2-LIF-EGFP.

Construction and identification of pIRES2-NGF-VEGF165 bicistronic eukaryotic expression vector

B. N. Li, Li, W. D., Feng, H. G., Lin, J. T., and Yuan, Z. Q., Construction and identification of pIRES2-NGF-VEGF165 bicistronic eukaryotic expression vector, vol. 13, pp. 5674-5685, 2014.

We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-NGF-VEGF165. The nerve growth factor (NGF) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NGF cDNA fragment was inserted into the multiple cloning sites of the pIRES2-EGFP vector to generate the bicistronic eukaryotic expression plasmid pIRES2-NGF-EGFP.

Genetic features of a translation initiation system composed of IRES element, nucleotide context surrounding the initiation codon, and translation initiation region of classical swine fever virus RNA

X. - X. Ma, Feng, Y. - P., Zhao, Y. - Q., Liu, J. - L., Chen, L., Guo, P. - H., Guo, J. - Z., Ma, L. - Y., and Ma, Z. - R., Genetic features of a translation initiation system composed of IRES element, nucleotide context surrounding the initiation codon, and translation initiation region of classical swine fever virus RNA, vol. 13, pp. 10803-10810, 2014.

Nucleotide and codon usage are typically examined to investigate viral evolution. In this study, we analyzed the genetic information of 46 strains of classical swine fever virus (CSFV) RNA, nucleotide usage in the internal ribosome entry site (IRES), the nucleotide context surrounding the initiation codon, and synonymous codon usage in the translation initiation region. Phylogenetic analysis of the IRES element indicated that the genetic diversity of this element is generally similar to the phylogenetic clusters of CSFV genotypes.

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