Internal transcribed spacer

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

Y. Y. Zhang, Huang, N., Xiao, X. H., Huang, L., Liu, F., Su, W. H., and Que, Y. X., Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers, vol. 14, pp. 7894-7909, 2015.

Sugarcane smut caused by the fungus Sporisorium scita­mineum is a worldwide disease and also one of the most prevalent diseases in sugarcane production in mainland China. To study molecular variation in S. scitamineum, 23 S. scitamineum isolates from the 6 primary sugar­cane production areas in mainland, China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, and Jiangxi Provinces), were assessed using internal tran­scribed spacer (ITS) methods.

Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species

Y. T. Liu, Chen, R. K., Lin, S. J., Chen, Y. C., Chin, S. W., Chen, F. C., and Lee, C. Y., Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species, vol. 13, pp. 2709-2717, 2014.

The Orchidaceae is one of the largest and most diverse families of flowering plants. The Dendrobium genus has high economic potential as ornamental plants and for medicinal purposes. In addition, the species of this genus are able to produce large crops. However, many Dendrobium varieties are very similar in outward appearance, making it difficult to distinguish one species from another. This study demonstrated that the 12 Dendrobium species used in this study may be divided into 2 groups by internal transcribed spacer (ITS) sequence analysis.

Delimiting invasive Myriophyllum aquaticum in Kashmir Himalaya using a molecular phylogenetic approach

M. A. Shah, Ali, M. A., Al-Hemaid, F. M., and Reshi, Z. A., Delimiting invasive Myriophyllum aquaticum in Kashmir Himalaya using a molecular phylogenetic approach, vol. 13, pp. 7564-7570, 2014.

Myriophyllum aquaticum (Vell.) Verdc. (family Haloragaceae) is one of the most invasive and destructive South American aquatic plant species and is present in a wide range of geographic regions, including the Kashmir Himalaya. Confusion regarding the taxonomic delimitation of M. aquaticum in the Himalayan region impedes effective and targeted management. Hence, our goal was improve the identification of M. aquaticum for exclusive delimitation from other related species in the study region using a molecular phylogenetic approach.

Molecular phylogenetic relationships among members of the family Phytolaccaceae sensu lato inferred from internal transcribed spacer sequences of nuclear ribosomal DNA

J. Lee, Kim, S. Y., Park, S. H., and Ali, M. A., Molecular phylogenetic relationships among members of the family Phytolaccaceae sensu lato inferred from internal transcribed spacer sequences of nuclear ribosomal DNA, vol. 12, pp. 4515-4525, 2013.

The phylogeny of a phylogenetically poorly known family, Phytolaccaceae sensu lato (s.l.), was constructed for resolving conflicts concerning taxonomic delimitations. Cladistic analyses were made based on 44 sequences of the internal transcribed spacer of nuclear ribosomal DNA from 11 families (Aizoaceae, Basellaceae, Didiereaceae, Molluginaceae, Nyctaginaceae, Phytolaccaceae s.l., Polygonaceae, Portulacaceae, Sarcobataceae, Tamaricaceae, and Nepenthaceae) of the order Caryophyllales.

Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers

P. P. Batista, Santos, J. F., Oliveira, N. T., Pires, A. P. D., Motta, C. M. S., and Lima, E. A. Luna- Alves, Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers, vol. 7, pp. 706-717, 2008.

The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification is nowadays performed by traditional techniques combined with molecular markers, resulting in a higher efficiency of isolate characterization.

Genetic variability in Melipona quinquefasciata (Hymenoptera, Apidae, Meliponini) from northeastern Brazil determined using the first internal transcribed spacer (ITS1)

J. O. P. Pereira, Freitas, B. M., Jorge, D. M. M., Torres, D. C., Soares, C. E. A., and Grangeiro, T. B., Genetic variability in Melipona quinquefasciata (Hymenoptera, Apidae, Meliponini) from northeastern Brazil determined using the first internal transcribed spacer (ITS1), vol. 8, pp. 641-648, 2009.

Melipona quinquefasciata is a ground-nesting South American stingless bee whose geographic distribution was believed to comprise only the central and southern states of Brazil. We obtained partial sequences (about 500-570 bp) of first internal transcribed spacer (ITS1) nuclear ribosomal DNA from Melipona specimens putatively identified as M. quinquefasciata collected from different localities in northeastern Brazil.

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