Invariant chain

N-terminal functional region of the invariant chain efficiently targets the binding of a CTL epitope to MHC class I molecules during cross-presentation

C. Wu, Zhang, D. G., Chen, F. F., Liu, X. L., Liu, S. J., and Yu, W. Y., N-terminal functional region of the invariant chain efficiently targets the binding of a CTL epitope to MHC class I molecules during cross-presentation, vol. 13, pp. 2438-2450, 2014.

Cross-presentation (CP) is important for priming T cell responses to many viral, bacterial, and tumor antigens. Here, we designed two Ii mutants, based on evidence that the invariant chain (Ii, also named CD74) binds newly synthesized MHC class I molecules with the class II-associated invariant chain peptide (CLIP) region of Ii, which occupies the peptide-binding groove.

Molecular cloning and site-directed mutagenesis of leucine-based sorting motifs of the porcine invariant chain

F. Z. Xu, Dai, Y., Li, L. M., Ding, X. L., and Yu, W. Y., Molecular cloning and site-directed mutagenesis of leucine-based sorting motifs of the porcine invariant chain, vol. 12, pp. 4489-4499, 2013.

Invariant chain (Ii) is a transmembrane protein that associates with MHC class II molecules in the endoplasmic reticulum. The cytoplasmic tail of Ii contains two leucine residues able to direct Ii to the endocytic pathway. We obtained the pig Ii gene by RT-PCR. Mutated Ii was prepared via site directed mutagenesis by the PCR Megaprimer method to study the effect of the two leucines on the localization of pig Ii. These mutated fragments were ligated to the vector pmCherry-C1. The recombinant plasmids were transiently transfected into COS-7 cells with LipofectamineTM 2000.

Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata)

S. J. Liu, Chen, F. F., Dai, Y., Wu, C., Ni, Q. S., and Yu, W. Y., Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata), vol. 10, pp. 2867-2880, 2011.

The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of cDNA ends (RACE)-PCR and by measuring expression of the MDIi gene in various tissues by real-time quantitative PCR. A full-length cDNA sequence of MDIi was obtained, 1188-bp long, encoding a 222-amino acid protein.

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