SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation
Ion implantation, a new biophysically mutagenic technique, has shown great potential for crop breeding. To reveal the mutation effect of low-energy ion implantation on Baiyangdian red lotus, sequence-related amplified polymorphism markers were used to amplify and detect the DNA sequence differences in mutants induced by Fe+ ion implantation. A total of 121 primer combinations were tested in 6 mutants and a control.