Knock out

A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency

L. H. Zhao, Zhao, Y. H., Liang, H., Yun, T., Han, X. J., Zhang, M. L., Zhou, X., Hou, D. X., Li, R. F., and Li, X. L., A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency, vol. 14, pp. 2750-2761, 2015.

With the development of gene targeting approaches, genomic mutation technologies in livestock animals such as gene trapping, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats and their associated systems have been improved. Although ZFNs have been used for gene targeting in many species, the off-target sites are still present. Using gene trapping, the workload of screening of targeted clones was decreased by generating a smaller number of drug-resistant clones.

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