Lemon balm

DNA isolation protocol for the medicinal plant lemon balm (Melissa officinalis, Lamiaceae)

S. Ghaffariyan, Mohammadi, S. A., and Aharizad, S., DNA isolation protocol for the medicinal plant lemon balm (Melissa officinalis, Lamiaceae), vol. 11, pp. 1049-1057, 2012.

Lemon balm (Melissa officinalis) is a medicinal plant that is widely used as a sedative or calmant, spasmolytic and antibacterial agent and sleep aid. This has led to a high demand for lemon balm products, resulting in the extinction of this species in some of its natural habitats. Molecular techniques have increasingly been used in plant diversity conservation and isolation of PCR amplifiable genomic DNA is an important pre-requisite. Lemon balm contains high levels of polyphenols and polysaccharides, which pose a major challenge for the isolation of high-quality DNA.

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