Lily

Reference gene selection for gene expression studies in lily using quantitative real-time PCR

M. F. Zhang, Liu, Q., Jia, G. X., Zhang, M. F., Liu, Q., and Jia, G. X., Reference gene selection for gene expression studies in lily using quantitative real-time PCR, vol. 15, p. -, 2016.

Quantitative real-time polymerase chain reaction (qRT-PCR) is an important technology used to analyze gene-expression levels. Reference genes, which are assumed to be expressed consistently across various developmental stages and in different tissues, were selected for expression level analysis. Using digital gene expression technology, we selected nine reference genes (18S, EF, CYCOL, SAND, GAPDH, ACTIN, BHLH, TIP, and Clathrin) as candidate reference genes for further study.

Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers

G. F. Cui, Wu, L. F., Wang, X. N., Jia, W. J., Duan, Q., Ma, L. L., Jiang, Y. L., and Wang, J. H., Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers, vol. 13, pp. 5778-5786, 2014.

Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average of 13 bands were amplified by each primer. Two software packages, POPGENE 1.32 and NTSYSpc 2.1, were used to analyze the data matrix.

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