Liver regeneration

14-3-3 Gene expression in regenerating rat liver after 2/3 partial hepatectomy

D. M. Xue, Guo, X. Q., Chen, R., Niu, Z. P., and Xu, C. S., 14-3-3 Gene expression in regenerating rat liver after 2/3 partial hepatectomy, vol. 14, pp. 2023-2030, 2015.

14-3-3 Proteins are a ubiquitous family of molecules that participate in protein kinase signaling pathways in all eukaryotic cells. Functioning as phosphoserine/phosphothreonine-binding modules, 14-3-3 proteins participate in the phosphorylation-dependent protein-protein interactions that control progression through the cell cycle, initiation and maintenance of DNA damage checkpoints, activation of MAP kinases, prevention of apoptosis, and coordination of integrin signaling and cytoskeletal dynamics.

MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG

J. J. Li, Chan, W. H., Leung, W. Y., Wang, Y., and Xu, C. S., MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG, vol. 14, pp. 4150-4160, 2015.

Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequencing. Their role toward rat normal hepatocyte line BRL-3A was studied by gain- and loss-of-function analyses, and one of them, microRNA-21 (miR-21), obviously upregulated and promoted BRL-3A cell proliferation.

Expression profiles uncover the relevance between colony stimulating factor-mediated signaling pathways in liver cells and partial hepatectomy-induced hepatic regeneration

X. G. Chen and Xu, C. S., Expression profiles uncover the relevance between colony stimulating factor-mediated signaling pathways in liver cells and partial hepatectomy-induced hepatic regeneration, vol. 13, pp. 6356-6366, 2014.

Colony stimulating factors (CSF) have been considered to modulate liver regeneration (LR) after partial hepatectomy (PH) at the tissue level. However, it remains unclear about precise mechanism of action of CSF in regeneration at the cellular level. Therefore, eight rat liver cell types were isolated by Percoll gradient centrifugation and magnetic beads. CSF-mediated signaling pathway genes were obtained by searching the related pathway databases and their expression profiles in 8 hepatic cell types were measured using rat Genome 230 2.0 Microarray.

Cloning and prokaryotic expression of rat homolog of Serpina3n and its expression change during liver regeneration

G. P. Wang, Zhang, X. S., Li, Y. H., Zheng, J. L., Tang, C. Z., and Zhang, W. X., Cloning and prokaryotic expression of rat homolog of Serpina3n and its expression change during liver regeneration, vol. 11, pp. 3175-3185, 2012.

A strikingly upregulated expressed sequence tag was screened from regenerating rat liver at 8 h in a 0-4-8-12 h short-interval successive partial hepatectomy model from a previous study. In the present study, a full-length open reading frame (ORF) corresponding to this expressed sequence tag was predicted through electronic cloning and was subsequently cloned from an 8-h rat regenerating liver and deposited in GenBank (accession No. HM448398). Sequence analysis of HM448398 and the predicted ORF revealed that the two ORFs may be different transcripts of a gene.

Impact of cold ischemia on cytokines after partial liver transplantation in rats

Q. - A. Qi, Yang, Z. - Y., Ma, K. - S., Lu, Q., Wang, S. - G., Li, X. - W., Xia, F., Liu, W., and Bie, P., Impact of cold ischemia on cytokines after partial liver transplantation in rats, vol. 12, pp. 4003-4008, 2013.

To study the impact of cold ischemia on tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) expression after liver transplantation, a stable model of partial liver transplantation in rats was established. The experimental animals were divided into the following groups: a partial hepatectomy control group, a group that received partial liver transplantation after 30 min of cold ischemia (experimental group A), and a group that received a partial liver transplantation after 10 h of cold ischemia (experimental group B). The survival rate was observed in each group.

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