Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate
“Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate”, vol. 15, p. -, 2016.
, This study investigated the efficacy and feasibility of inducing the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro using Sprague Dawley rats, as a model of hepatocyte generation for cell transplantation. BMSCs were isolated and grown using the adherent method and exposed to 5 or 10% liver tissue homogenate, before being collected for analysis after 0, 7, 14, and 21 days. Immunofluorescence and western blotting were employed to detect the liver-specific markers a-fetoprotein (AFP) and albumin (ALB).