Malayan gaur

Species identification of Malayan Gaur, Kedah-Kelantan and Bali cattle using polymerase chain reaction-restricted fragment length polymorphism

S. M. N. Romaino, Fazly-Ann, Z. A., Loo, S. S., Hafiz, M. M., Hafiz, M. D., Iswadi, M. I., Kashiani, P., Rosli, M. K. A., Syed-Shabthar, S. M. F., Md-Zain, B. M., and Abas-Mazni, O., Species identification of Malayan Gaur, Kedah-Kelantan and Bali cattle using polymerase chain reaction-restricted fragment length polymorphism, vol. 13, pp. 406-414, 2014.

Mitochondrial DNA (mtDNA) is a useful genetic marker that can be used for species identification. The cytochrome b (Cyt b) gene is a suitable mtDNA candidate gene for use in phylogenetic analyses due to its sequence variability, which makes it appropriate for comparisons at the subspecies, species, and genus levels. This study was conducted to develop a rapid molecular method for species identification of Malayan gaur (Bos gaurus hubbacki), Kedah-Kelantan (KK) (Bos indicus), and Bali (Bos javanicus) cattle in Malaysia.

Phylogenetic relationships of Malayan gaur with other species of the genus Bos based on cytochrome b gene DNA sequences

M. K. A. Rosli, Zakaria, S. S., Syed-Shabthar, S. M. F., Zainal, Z. Z., Shukor, M. N., Mahani, M. C., Abas-Mazni, O., and Md-Zain, B. M., Phylogenetic relationships of Malayan gaur with other species of the genus Bos based on cytochrome b gene DNA sequences, vol. 10, pp. 482-493, 2011.

The Malayan gaur (Bos gaurus hubbacki) is one of the three subspecies of gaurs that can be found in Malaysia. We examined the phylogenetic relationships of this subspecies with other species of the genus Bos (B. javanicus, B. indicus, B. taurus, and B. grunniens). The sequence of a key gene, cytochrome b, was compared among 20 Bos species and the bongo antelope, used as an outgroup. Phylogenetic reconstruction was employed using neighbor joining and maximum parsimony in PAUP and Bayesian inference in MrBayes 3.1.

Optimization of PCR conditions to amplify Cyt b, COI and 12S rRNA gene fragments of Malayan gaur (Bos gaurus hubbacki) mtDNA

M. K. A. Rosli, Zamzuriada, A. S., Syed-Shabthar, S. M. F., Mahani, M. C., Abas-Mazni, O., and Md-Zain, B. M., Optimization of PCR conditions to amplify Cyt b, COI and 12S rRNA gene fragments of Malayan gaur (Bos gaurus hubbacki) mtDNA, vol. 10. pp. 2554-2568, 2011.

PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various trials and combinations were tested to determine the best conditions of PCR mixture and profile to obtain the best PCR products for sequencing purposes.

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