Malaysia

IL-10 and IL-12B gene polymorphisms in a multiethnic Malaysian population

S. S. Sam, Teoh, B. T., and AbuBakar, S., IL-10 and IL-12B gene polymorphisms in a multiethnic Malaysian population, vol. 14, pp. 3257-3263, 2015.

Inheritance of polymorphisms in the interleukin (IL)-10 promoter and IL-12B genes, which influence cytokine production and activities, may define the balance in T helper response in infection and autoimmune diseases. In the present study, we investigated the distribution of the IL-10 promoter and IL-12B gene polymorphisms in a multiethnic Malaysian population.

Utility of DNA barcoding for rapid and accurate assessment of bat diversity in Malaysia in the absence of formally described species

J. - J. Wilson, Sing, K. - W., Halim, M. R. A., Ramli, R., Hashim, R., and Sofian-Azirun, M., Utility of DNA barcoding for rapid and accurate assessment of bat diversity in Malaysia in the absence of formally described species, vol. 13. pp. 920-925, 2014.

Bats are important flagship species for biodiversity research; however, diversity in Southeast Asia is considerably underestimated in the current checklists and field guides. Incorporation of DNA barcoding into surveys has revealed numerous species-level taxa overlooked by conventional methods. Inclusion of these taxa in inventories provides a more informative record of diversity, but is problematic as these species lack formal description. We investigated how frequently documented, but undescribed, bat taxa are encountered in Peninsular Malaysia.

Angiotensin-converting enzyme gene I/D dimorphism does not play a major role in the susceptibility of Malaysian systemic lupus erythematosus patients

L. - H. Lian, Lau, T. - P., Ching, A. - S., and Chua, K. - H., Angiotensin-converting enzyme gene I/D dimorphism does not play a major role in the susceptibility of Malaysian systemic lupus erythematosus patients, vol. 11, pp. 863-871, 2012.

Systemic lupus erythematosus (SLE) is an autoimmune disease that causes systemic damage, involving auto-reactive antibodies and over-deposition of immune complexes. Susceptibility to SLE is believed to be multifactorial, and genetics is one of the proven etiological factors; it can affect SLE development, severity and prognosis. We investigated a possible association between the angiotensin-converting enzyme gene and susceptibility to SLE in the Malaysian population. PCR was employed for the determination of I/D dimorphism of this gene.

Lack of association between TYK2 and STAT3 genes and Crohn’s disease in the Malaysian population

L. H. Lian, Lau, T. P., Lee, V. L., Lee, W. S., Hilmi, I., Goh, K. L., and Chua, K. H., Lack of association between TYK2 and STAT3 genes and Crohn’s disease in the Malaysian population, vol. 12, pp. 167-174, 2013.

This study aimed to investigate the potential association of TYK2 and STAT3 genes with the susceptibility to Crohn’s disease (CD) among Malaysians. DNA samples were obtained from 80 CD patients and 100 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism methods were employed for genotyping, followed by statistical analysis. In our current study, none of the single nucleotide polymorphisms of either TYK2 or STAT3 was statistically associated with the susceptibility to CD in our local population (P > 0.05).

Specific and straightforward molecular investigation of β-thalassemia mutations in the Malaysian Malays and Chinese using direct TaqMan genotyping assays

S. L. Kho, Chua, K. H., George, E., and Tan, J. A. M. A., Specific and straightforward molecular investigation of β-thalassemia mutations in the Malaysian Malays and Chinese using direct TaqMan genotyping assays, vol. 12, pp. 2409-2415, 2013.

Beta-thalassemia is a life-threatening inherited blood disorder. Rapid characterization of β-globin gene mutations is necessary because of the high frequency of Malaysian β-thalassemia carriers. A combination real-time polymerase chain reaction genotyping assay using TaqMan probes was developed to confirm β-globin gene mutations. In this study, primers and probes were designed to specifically identify 8 common β-thalassemia mutations in the Malaysian Malay and Chinese ethnic groups using the Primer Express software.

Lack of association between RANTES-28, SDF-1 gene polymorphisms and systemic lupus erythematosus in the Malaysian population

L. - H. Lian, Kee, B. - P., Ng, H. - L., and Chua, K. - H., Lack of association between RANTES-28, SDF-1 gene polymorphisms and systemic lupus erythematosus in the Malaysian population, vol. 10, pp. 2841-2850, 2011.

Regulated on activation, normal T-cell expressed and secreted (RANTES) and stromal cell-derived factor 1 (SDF-1) are members of the CC- and CXC-chemokine families, respectively. Both genes have been postulated to be involved in the pathogenesis of systemic lupus erythematosus (SLE). We analyzed position 28 of the RANTES gene promoter region, as well as the SNP observed in the 3' UTR of the SDF-1 gene at position 801, in 130 patients presenting SLE at the Malaya University Medical Centre. Screening of 130 healthy volunteer controls using RFLP was also performed.

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