Mango peel

Evaluation of four protein extraction methods for proteomic analysis of mango peel

D. J. Liao, Lu, X. P., Chen, H. S., Lu, Y., Mo, Z. Y., Liao, D. J., Lu, X. P., Chen, H. S., Lu, Y., and Mo, Z. Y., Evaluation of four protein extraction methods for proteomic analysis of mango peel, vol. 15, p. -, 2016.

The peel of mango (Mangifera indica L.) is a special plant tissue that contains many compounds that interfere with protein extraction. A successful separation with Two-dimensional electrophoresis (2-DE) is the key step for proteomic analysis. To evaluate the efficiencies of mango peel protein extraction for 2-DE, four extraction methods were tested: 1) 2-D clean-up kit, 2) trichloroacetic acid/acetone precipitation, 3) phenol extraction, 4) phenol with methanol/ammonium acetate precipitation.

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