Mesenchymal stem cells

Identification of long non-coding RNA involved in osteogenic differentiation from mesenchymal stem cells using RNA-Seq data

W. Q. Song, Gu, W. Q., Qian, Y. B., Ma, X., Mao, Y. J., and Liu, W. J., “Identification of long non-coding RNA involved in osteogenic differentiation from mesenchymal stem cells using RNA-Seq data”, vol. 14, pp. 18268-18279, 2015.

The aim of this study was to identify long non-coding RNA (lncRNA) associated with osteogenic differentiation from mesenchymal stem cells (MSCs) using high-throughput RNA sequencing (RNA-Seq) data. RNA-Seq dataset was obtained from the European Bioinformatics Institute (accession No. PRJEB4496), including two replicates each for immortalized mesenchymal stem cells iMSC#3 cultured in growth medium (GM) and differentiation medium (DM) for 28 days. The clean reads were aligned to a hg19 reference genome by Tophat and assembled by Cufflinks to identify the known and novel transcripts.

Genetic expression and functional characterization of the RUNX2 gene in human adult bone marrow mesenchymal stem cells

J. Y. Zhang and Li, L. C., “Genetic expression and functional characterization of the RUNX2 gene in human adult bone marrow mesenchymal stem cells”, vol. 14, pp. 18210-18217, 2015.

Past studies have revealed the critical role of runt-related transcription factor 2 (RUNX2) in the proliferation and differentiation of mesenchymal stem cells (MSCs). This study therefore aimed to investigate the expression profile of the RUNX2 gene in human bone marrow MSCs and its biological characteristics. Bone marrow MSCs were separated from 12 patients who had received hip joint replacement surgery.

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