methodology

Heteroduplex formation and S1 digestion for mapping alternative splicing sites

E. N. Ferreira, Rangel, M. C. R., Pineda, P. B., Vidal, D. O., Camargo, A. A., Souza, S. J., and Carraro, D. M., Heteroduplex formation and S1 digestion for mapping alternative splicing sites, vol. 7, pp. 958-969, 2008.

The identification of alternatively spliced transcripts has contributed to a better comprehension of developmental mechanisms, tissue-specific physiological processes and human diseases. Polymerase chain reaction amplification of alternatively spliced variants commonly leads to the formation of heteroduplexes as a result of base pairing involving exons common between the two variants. S1 nuclease cleaves single-stranded loops of heteroduplexes and also nicks the opposite DNA strand.

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