Heteroduplex formation and S1 digestion for mapping alternative splicing sites
“Heteroduplex formation and S1 digestion for mapping alternative splicing sites”, vol. 7, pp. 958-969, 2008.
, The identification of alternatively spliced transcripts has contributed to a better comprehension of developmental mechanisms, tissue-specific physiological processes and human diseases. Polymerase chain reaction amplification of alternatively spliced variants commonly leads to the formation of heteroduplexes as a result of base pairing involving exons common between the two variants. S1 nuclease cleaves single-stranded loops of heteroduplexes and also nicks the opposite DNA strand.