Microsatellite

Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology

L. M. Li, Wei, L., Jiang, H. Y., Zhang, Y., Zhang, X. J., Yuan, L. H., and Chen, J. P., “Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology”, vol. 14, pp. 7910-7913, 2015.

Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more infor­mation on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the develop­ment of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of ob­served alleles per locus is 6.6 (ranging from 2 to 17).

Development and characterization of microsatellite markers for molecular genetic diversity in Atrina pectinata

Y. F. Ning, Li, Z. B., Shangguan, J. B., Huang, Y. S., Jiang, P. D., Xu, A. L., and Yuan, Y., “Development and characterization of microsatellite markers for molecular genetic diversity in Atrina pectinata”, vol. 14, pp. 8290-8293, 2015.

The pen shell, Atrina pectinata, is an economically valuable species that is widely distributed along the coastal waters of temperate and tropical areas, mainly growing in the Indian and Pacific Oceans. Eight novel microsatellite loci from the genome of A. pectinata were developed using fast isolation by amplified fragment length polymorphism of sequence containing repeats. The loci were screened in 30 wild individuals.

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