Microsatellite marker

Evaluation of genetic variability to form heterotic groups in popcorn

C. Vittorazzi, Júnior, A. T. Amaral, Guimarães, A. G., Silva, F. H. L., Pena, G. F., Daher, R. F., Gerhardt, I. F. S., Oliveira, G. H. F., Santos, P. H. A. D., Souza, Y. P., Kamphorst, S. H., and Lima, V. J., “Evaluation of genetic variability to form heterotic groups in popcorn”, Genetics and Molecular Research, vol. 17, no. 3, 2018.

Development of microsatellite markers in the tetraploid fern Ceratopteris thalictroides (Parkeriaceae) using RAD tag sequencing

X. Y. Yang, Long, Z. C., Gichira, A. W., Guo, Y. H., Wang, Q. F., Chen, J. M., Yang, X. Y., Long, Z. C., Gichira, A. W., Guo, Y. H., Wang, Q. F., and Chen, J. M., “Development of microsatellite markers in the tetraploid fern Ceratopteris thalictroides (Parkeriaceae) using RAD tag sequencing”, vol. 15, p. -, 2016.

To understand the genetic variability of the tetraploid fern Ceratopteris thalictroides (Parkeriaceae), we described 30 polymorphic microsatellite markers obtained using the restriction site-associated DNA (RAD) tag sequencing technique. A total of 26 individuals were genotyped for each marker. The number of alleles per locus ranged from 4 to 10, and the expected heterozygosity and the Shannon-Wiener index ranged from 0.264 to 0.852 and 0.676 to 2.032, respectively.

Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin

Y. - X. Liu, Ma, C. - Y., Ma, H. - Y., Feng, C. - L., Li, S. - J., and Ma, L. - B., “Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin”, vol. 14, pp. 15320-15324, 2015.

Nine microsatellite DNA markers were developed and characterized for Siganus oramin by the 5'-anchored polymerase chain reaction technique. A total of 42 alleles were identified in 30 individuals, and the number of alleles per locus ranged from 3 to 7, with an average of 4.7. The observed and expected heterozygosity per locus ranged from 0.5333 to 1.0000 and from 0.5254 to 0.8474, respectively, with an average of 0.7422 and 0.6906, respectively.

Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus

L. Lin, Li, C. H., Xu, S. N., Liu, Y., and Xiao, Y. Y., “Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus”, vol. 14, pp. 10944-10947, 2015.

We isolated and characterized 22 polymorphic microsatellite loci in the crimson snapper (Lutjanus erythropterus) using a (GT)13-enriched genomic library. We found three to 15 alleles per locus, with a mean of 6.68. The observed and expected heterozygosities ranged from 0.087 to 0.978 and from 0.125 to 0.904, respectively, with averages of 0.576 and 0.650, respectively. Only three loci showed significant deviation from the Hardy-Weinberg equilibrium after Bonferroni correction. Four loci showed evidence for null alleles.

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