Microsatellites

Novel and highly informative Capsicum SSR markers and their cross-species transferability

G. S. C. Buso, Reis, A. M. M., Amaral, Z. P. S., Ferreira, M. E., Buso, G. S. C., Reis, A. M. M., Amaral, Z. P. S., and Ferreira, M. E., Novel and highly informative Capsicum SSR markers and their cross-species transferability, vol. 15, p. -, 2016.

This study was undertaken primarily to develop new simple sequence repeat (SSR) markers for Capsicum. As part of this project aimed at broadening the use of molecular tools in Capsicum breeding, two genomic libraries enriched for AG/TC repeat sequences were constructed for Capsicum annuum. A total of 475 DNA clones were sequenced from both libraries and 144 SSR markers were tested on cultivated and wild species of Capsicum. Forty-five SSR markers were randomly selected to genotype a panel of 48 accessions of the Capsicum germplasm bank.

Genetic structure of the threatened Hopea chinensis in the Quang Ninh Province, Vietnam

N. T. P. Trang, Triest, L., Trang, N. T. P., and Triest, L., Genetic structure of the threatened Hopea chinensis in the Quang Ninh Province, Vietnam, vol. 15, p. -, 2016.

Hopea chinensis Hand-Mazz (synonym H. hongayensis Tardieu), is a wind and insect pollinated species. It is a threatened species known only from two locations: Quang Ninh (Vietnam) and Guangxi (China). As an endemic species, it is worth preserving both for dipterocarp biodiversity, as well as for its medicinal use and economic importance as a fine wood. The genetic diversity and population genetic structure of H. chinensis was investigated, using natural populations distributed throughout the Ba Mun and Cai Lim islands, Quang Ninh Province, Vietnam.

First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers

H. Y. Ma, Li, S. J., Feng, N. N., Ma, C. Y., Wang, W., Chen, W., Ma, L. B., Ma, H. Y., Li, S. J., Feng, N. N., Ma, C. Y., Wang, W., Chen, W., and Ma, L. B., First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers, vol. 15, p. -, 2016.

The mud crab (Scylla paramamosain) is of economic importance for the fisheries and aquaculture industry in China. In this study, we constructed the first genetic linkage map for this species using microsatellite and amplified fragment length polymorphism (AFLP) markers. The map consisted of 65 linkage groups, including 34 triplets and 9 doublets. A total of 212 molecular markers were mapped, including 60 microsatellites and 152 AFLP markers. The linkage groups ranged from 7 to 102.5 cM and covered 2746.4 cM in length.

Isolation and characterization of microsatellite markers in Ruditapes philippinarum

Z. B. Li, Ning, Y. F., Shangguan, J. B., Li, B. B., Mao, X. Q., Huang, Y. S., Yuan, Y., Li, Z. B., Ning, Y. F., Shangguan, J. B., Li, B. B., Mao, X. Q., Huang, Y. S., and Yuan, Y., Isolation and characterization of microsatellite markers in Ruditapes philippinarum, vol. 15, p. -, 2016.

Ruditapes philippinarum is considered a commercially valuable species, which is commonly found in tidal flats along West Pacific coasts. In China, it is mainly distributed in the southeast sea. In this study, 16 novel microsatellite loci from the R. philippinarum genome were developed, using the protocol of fast isolation by amplified fragment length polymorphism of sequence containing repeats. Thirty-two wild-caught individuals were used to evaluate the degree of polymorphism of these markers.

Structure and genetic diversity of natural Brazilian pepper populations (Schinus terebinthifolius Raddi)

S. V. Álvares-Carvalho, Duarte, J. F., Santos, T. C., Santos, R. M., Silva-Mann, R., Carvalho, D., Álvares-Carvalho, S. V., Duarte, J. F., Santos, T. C., Santos, R. M., Silva-Mann, R., and Carvalho, D., Structure and genetic diversity of natural Brazilian pepper populations (Schinus terebinthifolius Raddi), vol. 15, p. -, 2016.

In the face of a possible loss of genetic diversity in plants due the environmental changes, actions to ensure the genetic variability are an urgent necessity. The extraction of Brazilian pepper fruits is a cause of concern because it results in the lack of seeds in soil, hindering its distribution in space and time. It is important to address this concern and explore the species, used by riparian communities and agro-factories without considering the need for keeping the seeds for natural seed banks and for species sustainability.

Isolation and characterization of eight novel microsatellite markers in Acanthopagrus schlegelii

X. Q. Mao, Li, Z. B., Yuan, Y., Ning, Y. F., Shangguan, J. B., Huang, Y. S., Yang, M., Li, B. B., Mao, X. Q., Li, Z. B., Yuan, Y., Ning, Y. F., Shangguan, J. B., Huang, Y. S., Yang, M., and Li, B. B., Isolation and characterization of eight novel microsatellite markers in Acanthopagrus schlegelii, vol. 15, p. -, 2016.

Acanthopagrus schlegelii is a warm temperate demersal fish, which inhabits the sediment substrate or rocky reefs in shallow seas. As this fish is a nutritionally endowed species with good palatability, it is a highly valuable commercial species for aquaculture and has a long historical standing in Western Pacific countries. Because the population of this fish is currently declining in China, studies and measures aimed at addressing this decline are needed. In this study, eight microsatellite markers were screened from 30 wild A.

Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing

A. W. Gichira, Long, Z. C., Hu, G. W., Gituru, R. W., Wang, Q. F., Chen, J. M., Gichira, A. W., Long, Z. C., Hu, G. W., Gituru, R. W., Wang, Q. F., and Chen, J. M., Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing, vol. 15, p. -, 2016.

Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individuals. The average number of alleles per locus was 7.6, while the observed and expected heterozygosities ranged from 0.000 to 0.958 and from 0.354 to 0.883, respectively.

Isolation and characterization of microsatellite markers in Atrina vexillum Born

Y. F. Ning, Li, Z. B., Huang, Y. S., Mao, X. Q., Li, B. B., Yuan, Y., Shangguan, J. B., Ning, Y. F., Li, Z. B., Huang, Y. S., Mao, X. Q., Li, B. B., Yuan, Y., Shangguan, J. B., Ning, Y. F., Li, Z. B., Huang, Y. S., Mao, X. Q., Li, B. B., Yuan, Y., and Shangguan, J. B., Isolation and characterization of microsatellite markers in Atrina vexillum Born, vol. 15, p. -, 2016.

Atrina vexillum Born is an economically valuable species, widely distributed in the coastal waters of temperate and tropical areas of the Asia Pacific region. Twenty one novel microsatellite loci were identified in the genome of A. vexillum Born using the protocol for fast isolation by amplified fragment length polymorphism of sequence containing repeats. Thirty-two wild type individuals were used to evaluate the degree of polymorphism of these markers.

Isolation and characterization of novel microsatellites for Abies koreana and A. nephrolepis (Pinaceae)

J. K. Hong, Lim, J., Lee, B. Y., Kwak, M., Hong, J. K., Lim, J., Lee, B. Y., Kwak, M., Hong, J. K., Lim, J., Lee, B. Y., and Kwak, M., Isolation and characterization of novel microsatellites for Abies koreana and A. nephrolepis (Pinaceae), vol. 15, p. -, 2016.

Abies koreana is an endemic and rare species from Korea and is classified as endangered by the International Union for Conservation of Nature. Although the genetic diversity assessment for current population of A. koreana needs to be performed urgently, no microsatellite markers have been developed for this species. In the present study, we developed 22 novel polymorphic microsatellite loci and the characteristics of these loci were determined in A.

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