Molecular markers

Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers

C. A. Nogueira, Stafuzza, N. B., Ribeiro, T. P., Prado, A. D. L., Menezes, I. P. P., Peixoto, N., Gonçalves, P. J., and Almeida, L. M., Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers, vol. 14, pp. 15996-16005, 2015.

Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six botanic varieties that differ morphologically mainly in the shapes of their leaves and flowers.

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

E. T. Werner, Soares, T. C. B., Gontijo, A. B. P. L., Neto, J. D. Souza, and Amaral, J. A. Tdo, Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers, vol. 14, pp. 16450-16460, 2015.

Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar ‘FMS brilhante’ using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers.

Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle

Z. D. Zhao, Li, A. N., Wei, S. J., Wang, M. M., Li, S. J., and Zan, L. S., Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle, vol. 14, pp. 17439-17446, 2015.

Fatty acid transport protein 1 (FATP1), an integral membrane protein that facilitates long-chain fatty acid influx, is involved in the genetic network for oleic acid synthesis. The aim of this study was to examine the association of FATP1 polymorphisms with live animal meat quality traits in Chinese Qinchuan cattle. Quantitative real-time PCR analysis demonstrated that FATP1 has a broad tissue distribution in Qinchuan cattle and is highly expressed in longissimus dorsi muscle and back fat.

Genetic diversity of the Neotropical tree Hancornia speciosa Gomes in natural populations in Northeastern Brazil

H. J. Jimenez, Martins, L. S. S., Montarroyos, A. V. V., Junior, J. F. Silva, Alzate-Marin, A. L., and Filho, R. M. Moraes, Genetic diversity of the Neotropical tree Hancornia speciosa Gomes in natural populations in Northeastern Brazil, vol. 14, pp. 17749-17757, 2015.

Mangabeira (Hancornia speciosa Gomes) is a fruit tree of the Apocynaceae family, which is native to Brazil and is a very important food resource for human populations in its areas of occurrence. Mangabeira fruit is collected as an extractive activity, and no domesticated varieties or breeding programs exist. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil.

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

B. S. Hsie, Brito, J. Z., Nova, M. X. Vila, Borges-Paluch, L. R., Silva, M. V., and Donato, V. M. S. T., Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers, vol. 14, pp. 17651-17659, 2015.

Sugarcane culture is an important source of income for the Brazilian economy. The aim of this study was to identify somaclonal variation in sugarcane varieties RB943365 and RB92579 arising from micropropagation using inter-simple sequence repeat (ISSR) DNA markers. The evaluated plants were generated from the in vitro propagation of shoot tips grown in MS medium supplemented with vitamins, myoinositol, glycine, and sucrose, without the use of growth regulators.

Agro-ecological variations of sheath rot disease of rice caused by Sarocladium oryzae and DNA fingerprinting of the pathogen’s population structure

T. Islam M. Chowdhury, M. Mian, S., Mia, M. A. Taher, Rafii, M. Y., and Latif, M. A., Agro-ecological variations of sheath rot disease of rice caused by Sarocladium oryzae and DNA fingerprinting of the pathogen’s population structure, vol. 14, pp. 18140-18152, 2015.

To examine the impact of regional and seasonal variations on the incidence and severity of sheath rot, a major seed-borne disease of rice caused by Sarocladium oryzae, data on incidence and severity were collected from 27 selected fields in the Gazipur, Rangpur, Bogra, Chittagong, Comilla, Gopalgonj, Jessore, Manikgonj, and Bhola districts of Bangladesh in rain-fed and irrigated conditions.

ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding

A. G. R. Sousa, Souza, M. M., Melo, C. A. F., and Sodré, G. A., ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding, vol. 14, pp. 18534-18545, 2015.

Inter simple sequence repeat (ISSR) molecular markers were developed and used to investigate interspecific genetic variation in 25 wild species of Passiflora preserved in an active germplasm bank (BAG-Passifloras); intraspecific diversity was also analyzed in P. cincinnata accessions. Of 31 primers tested, 20 identified polymorphic loci with a total of 331 bands, suggesting high polymorphism in the sample. Interspecific polymorphism was greater than intraspecific polymorphism. This is a common finding in studies of genetic variation using dominant markers.

Populations of Erythrina velutina Willd. at risk of extinction

M. F. V. Melo, Gonçalves, L. O., Rabbani, A. R. C., Álvares-Carvalho, S. V., Pinheiro, J. B., Zucchi, M. I., and Silva-Mann, R., Populations of Erythrina velutina Willd. at risk of extinction, vol. 14, pp. 10298-10307, 2015.

The goal of this study was to characterize the structure of two natural populations of the coral tree using RAPD and ISSR markers. The study evaluated all individuals in two different areas in the northeastern region of Brazil: the first was in the riparian area, 10 km x 100 m along the edge of the lower São Francisco River, and the second was in the municipality of Pinhão, in a semiarid region between the municipalities of Neópolis and Santana do São Francisco. We used all the coral trees present in those two areas (37 individuals).

Molecular cytogenetic analysis of a ring-Y infertile male patient

F. M. Carvalho, Wolfgramm, E. V., Degasperi, I., Verbeno, B. M., Vianna, B. A., Chagas, F. F., Paula, F., Louro, I. D., and Perroni, A. M. S., Molecular cytogenetic analysis of a ring-Y infertile male patient, vol. 6. pp. 59-66, 2007.

In the present study, we report on the case of a 43-year-old male patient seeking for fertility assistance, who showed a seminal analysis and testicular biopsy of complete azoospermia. Peripheral blood culture for chromosome studies revealed a karyotype of 46 chromosomes with a ring-Y-chromosome that lost the long arm heterochromatin. Molecular analysis of genomic DNA from the patient detected the presence of the sex-determining region of the Y-chromosome (SRY) but the complete absence of regions involved in spermatogenesis (AZFa, AZFb, AZFc).

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