MSAP

Methylation-sensitive amplification polymorphism analysis of fat and muscle tissues in pigs

J. D. Ma, Li, M. Z., Zhou, S. L., Zhou, C. W., and Li, X. W., Methylation-sensitive amplification polymorphism analysis of fat and muscle tissues in pigs, vol. 11, pp. 3505-3510, 2012.

DNA methylation may be involved in regulating the expression of protein-coding genes, resulting in different fat and muscle phenotypes. Using a methylation-sensitive amplified polymorphism approach, we obtained 7423 bands by selective amplification of genomic DNA from six different fat depots and two heterogeneous muscle types from Duroc/Landrace/Yorkshire cross-bred pigs.

Inheritance of cytosine methylation patterns in purebred versus hybrid chicken lines

Q. Xu, Sun, D. X., Li, J. L., Liu, R., Wang, Y. C., and Zhang, Y., Inheritance of cytosine methylation patterns in purebred versus hybrid chicken lines, vol. 12, pp. 2674-2687, 2013.

We used methylation-sensitive amplified polymorphism to examine DNA methylation levels and CCGG patterns in parents and offsprings of 3 groups of adult chickens, purebred White Leghorn (AA), White Plymouth Rock (EE), and crossbred individuals (EA) using 10 primer combinations. We found that about 66% of the cytosines at CCGG sites were not methylated. Fully methylated sites were less frequent than hemi-methylated sites in the chicken genome; these frequencies were different from those of plants.

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