Multiplex PCR

Association of GSTM1 and GSTT1 with ageing in auto repair shop workers

S. A. Eshkoor, Marashi, S. J., Ismail, P., Rahman, S. A., Mirinargesi, M., Adon, M. Y., and Devan, R. V., Association of GSTM1 and GSTT1 with ageing in auto repair shop workers, vol. 11, pp. 1486-1496, 2012.

We evaluated the possible influence of glutathione S-transferase mu (GSTM1) and glutathione S-transferase theta (GSTT1) genes on genetic damage due to occupational exposure, which contributes to accelerate ageing. This study was conducted on 120 car auto repair workshop workers exposed to occupational hazards and 120 controls without this kind of exposure. The null and non-null genotypes of GSTM1 and GSTT1 genes were determined by multiplex PCR.

Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

F. S. Carvalho, Wenceslau, A. A., Teixeira, M., and Albuquerque, G. R., Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus, vol. 10, pp. 996-1005, 2011.

We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR.

Influence of glutathione S-transferase polymorphisms on type-2 diabetes mellitus risk

M. A. Amer, Ghattas, M. H., Abo-ElMatty, D. M., and Abou-El-Ela, S. H., Influence of glutathione S-transferase polymorphisms on type-2 diabetes mellitus risk, vol. 10, pp. 3722-3730, 2011.

Glutathione S-transferase (GST) protects cells against oxidative stress. We evaluated the effect of genetic polymorphisms of the GST gene family on the risk of developing type-2 diabetes mellitus and on glycemic control. We also investigated the effects of smoking combined with these polymorphisms on type-2 diabetes mellitus risk. We enrolled 100 type-2 diabetes mellitus patients and 100 healthy controls matched for age, gender and origin, from the Sinai area of Egypt. Fasting serum glucose, HbA1c and lipid profiles were determined.

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