Mutagenesis

Mutagenic potential of Cordia ecalyculata alone and in association with Spirulina maxima for their evaluation as candidate anti-obesity drugs

R. P. Araldi, Rechiutti, B. M., Mendes, T. B., Ito, E. T., and Souza, E. B., Mutagenic potential of Cordia ecalyculata alone and in association with Spirulina maxima for their evaluation as candidate anti-obesity drugs, vol. 13, pp. 5207-5220, 2014.

Obesity is one of the most important nutritional disorders, and can be currently considered as an epidemic. Although there are few weight reduction drugs available on the market, some new drug candidates have been proposed, including Cordia ecalyculata, a Brazilian plant with anorectic properties, and Spirulina maxima, a cyanobacterium with antioxidant and anti-genotoxic activity. In this study, we evaluated the mutagenic potential of C. ecalyculata at doses of 150, 300, and 500 mg/kg alone and in association with S.

Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants

A. Wannajindaporn, Poolsawat, O., Chaowiset, W., and Tantasawat, P. A., Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants, vol. 13, pp. 5333-5342, 2014.

In vitro mutagenesis of Dendrobium ‘Earsakul’ was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymorphic fragments were produced by 9 of 12 ISSR primers. A total of 173 amplified ISSR fragments varying in size from 140 to 5000 bp were obtained, 39 of which were polymorphic (22.5%).

Cytogenetic damage in the buccal epithelium of Brazilian aviators occupationally exposed to agrochemicals

L. B. Minasi, Costa, E. O. A., Silva, D. M., Melo, C. O. A., de Almeida, J. G., Vieira, T. C., Júnior, R. L. Silva, Ribeiro, C. L., da Silva, C. C., and da Cruz, A. D., Cytogenetic damage in the buccal epithelium of Brazilian aviators occupationally exposed to agrochemicals, vol. 10. pp. 3924-3929, 2011.

The frequency of micronuclei in both buccal cells and peripheral blood lymphocytes is extensively used as a biomarker of chromosomal damage and genome stability in human populations. We examined whether prolonged exposure to complex mixtures of pesticides leads to an increase in cytogenetic damage.

Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice

T. D. A. Costa, Vieira, S., Andrade, S. F., and Maistro, E. L., Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice, vol. 7, pp. 679-686, 2008.

Cattley guava (Psidium cattleyanum Sabine) is a native fruit of Brazil that is popular both as a sweet food and for its reputed therapeutic properties. We examined whether it could damage DNA using the alkaline single-cell gel electrophoresis (comet assay) and the micronucleus test in leukocytes and in bone marrow cells of mice. P. cattleyanum leaf extract was tested at concentrations of 1000, 1500 and 2000 mg/kg. N-nitroso-N-ethylurea was used as a positive control.

The anticancer homeopathic composite “Canova Method” is not genotoxic for human lymphocytes in vitro

I. C. Seligmann, Lima, P. D. L., Cardoso, P. C. S., Khayat, A. S., Bahia, M. O., Buchi, Dde Freitas, Cabral, I. R., and Burbano, R. R., The anticancer homeopathic composite “Canova Method” is not genotoxic for human lymphocytes in vitro, vol. 2, pp. 223-228, 2003.

The Canova Methodâ (CM) is a homeopathic medicine indicated for the treatment of patients with cancer and for pathologies that involve a depressed immune system, such as AIDS. This product is composed of homeopathic dilutions of Aconitum napellus, Arsenicum album (arsenic trioxide), Bryonia alba, Lachesis muta venom and Thuya occidentalis. It stimulates the immune system by activating macrophages.

RAPD analysis of herbicide-resistant Brasilian rice lines produced via mutagenesis

S. S. Sandhu, Bastos, C. R., Azini, L. Ernesto, Neto, A. Tulmann, and Colombo, C., RAPD analysis of herbicide-resistant Brasilian rice lines produced via mutagenesis, vol. 1, pp. 359-370, 2002.

Over the last two decades, mutational techniques have become one of the most important tools available to progressive rice- breeding programs. In a mutation-breeding program initiated in 1999 at the Instituto Agronômico of Campinas, SP, Brazil, a rice line, IAC103, was selected for mutational studies with gamma radiation and ethyl methyl sulfonate mutagenesis, with the aim of developing a herbicide-resistant crop. After mutagenesis, surviving plants were exposed to glufosinate to check for herbicide resistance, which was examined up to the second generation.

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