Mutagenesis

Improving production of extracellular proteases by random mutagenesis and biochemical characterization of a serine protease in Bacillus subtilis S1-4

X. C. Wang, Zhao, H. Y., Liu, G., Cheng, X. J., Feng, H., Wang, X. C., Zhao, H. Y., Liu, G., Cheng, X. J., and Feng, H., Improving production of extracellular proteases by random mutagenesis and biochemical characterization of a serine protease in Bacillus subtilis S1-4, vol. 15, p. -, 2016.

The feather is a valuable by-product with a huge annual yield produced by the poultry industry. Degradation of feathers by microorganisms is a prerequisite to utilize this insoluble protein resource. To improve the degrading efficiency of feathers, mutagenesis of the bacterium Bacillus subtilis S1-4 was performed. By combining ultraviolet irradiation and N-methyl-N’-nitro-N-nitrosoguanidine treatment for mutagenesis, a high protease-producing mutant (UMU4) of B.

Campomanesia adamantium extract induces DNA damage, apoptosis, and affects cyclophosphamide metabolism

M. D. Martello, David, N., Matuo, R., Carvalho, P. C., Navarro, S. D., Monreal, A. C. D., Cunha-Laura, A. L., Cardoso, C. A. L., Kassuya, C. A. L., Oliveira, R. J., Martello, M. D., David, N., Matuo, R., Carvalho, P. C., Navarro, S. D., Monreal, A. C. D., Cunha-Laura, A. L., Cardoso, C. A. L., Kassuya, C. A. L., Oliveira, R. J., Martello, M. D., David, N., Matuo, R., Carvalho, P. C., Navarro, S. D., Monreal, A. C. D., Cunha-Laura, A. L., Cardoso, C. A. L., Kassuya, C. A. L., and Oliveira, R. J., Campomanesia adamantium extract induces DNA damage, apoptosis, and affects cyclophosphamide metabolism, vol. 15, p. -, 2016.

Campomanesia adamantium (Cambess.) O. Berg. is originally from Brazil. Its leaves and fruits have medicinal properties such as anti-inflammatory, antidiarrheal and antiseptic properties. However, the mutagenic potential of this species has been reported in few studies. This study describes the mutagenic/antimutagenic, splenic phagocytic, and apoptotic activities of C. adamantium hydroethanolic extract with or without cyclophosphamide in Swiss mice.

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