Mutation

Analysis of specific sequences in mutant rice generated by introduction of exogenous corn DNA

W. C. Li, Ji, S. D., Tian, C. Z., Li, Z. K., Wang, X. C., Zhang, H. C., Sun, Y. L., Shi, X. Z., Wang, T. X., and Li, J. Y., Analysis of specific sequences in mutant rice generated by introduction of exogenous corn DNA, vol. 14, pp. 18980-18989, 2015.

Rice variation induced by the introduction of exogenous DNA has become an important method of improving rice varieties and creating new germplasms. In this study, we transferred maize genomic DNA fragments to the receptor of Nipponbare rice using a modified “pollen-tube pathway” method. Material from mutant rice B1 and B2 were acquired and 14 specific bands were obtained from the material using amplified fragment length polymorphism analysis. From the 14 specific sequences obtained, there were 3791 bp, including 144 base mutations with a base mutation rate of 3.80%.

Subscribe to Mutation